gerE

transcriptional regulator (repressor or activator) of a subset of [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]-dependent late spore coat genes

locus: BSU_28410

pI: 9.64

mw: 8.43 kDa

protein length: 74 aa

gene length: 225 bp

function: regulation of [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]-dependent gene expression

product: transcriptional regulator (LuxR-FixJ family)

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW 4.2.4.2|Additional germination proteins]

Gene

Coordinates: 2,904,727 2,904,951

Phenotypes of a mutant

• unable to germinate efficiently [Pubmed|3139490]

The protein

Protein family

• LuxR-FixJ family of transcription regulators

[SW|Domains]

• [SW|HTH luxR-type domain] (aa 5-70) (according to UniProt)

Structure

• [PDB|1FSE]

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000047 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]
  description: [Pubmed|3114423]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID]: activation, [Pubmed|7896717], in [regulon|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|SpoIIID regulon]
  • [protein|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|CitB]: mRNA binding, results in mRNA accumulation [Pubmed|16923907], in [regulon|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|CitB regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK], [SW|SpoIIID]) [Pubmed|15699190,7896717]

Biological materials

Mutant

• BKE28410 ([gene|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTATTGTAACCCTCCTT, downstream forward: _UP4_TAATCCTTGCCGGTATTCCT
• BKK28410 ([gene|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTATTGTAACCCTCCTT, downstream forward: _UP4_TAATCCTTGCCGGTATTCCT

labs

• [SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]
• [SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

References

Reviews

• 31350897

Original Publications

• 7519271,2474075,11418558,10400595,7592393,11741866,7592342,3135411,9852018,1691789,15383836,11150673,1518043,7814326,14523132,12031481,11418558,3114423,7896717,11243786,17890309,15470121,20435725,21670523,23064347,3139490,25259857,6787012,26279233,16923907