lplD

alpha-galacturonidase

locus: BSU_07130

pI: 5.36

mw: 49.32 kDa

protein length: 446 aa

gene length: 1341 bp

function: utilization of pectin

product: alpha-galacturonidase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.26|Utilization of pectin]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

Gene

Coordinates: 782,958 784,298

The protein

Catalyzed reaction/ biological activity

• cleavage of alpha-1,4-di-galacturonate [Pubmed|23416295]
• [(1→4)-α-D-galacturonosyl]_(n) + H₂O --> [(1→4)-α-D-galacturonosyl]_(n-1) + α-D-galacturonate (according to UniProt)

Protein family

• [SW|Glycosyl hydrolase 4 family] (according to UniProt)

[SW|Domains]

• active site motif: CHEV [Pubmed|23416295]

[SW|Cofactors]

• NAD, Mn(2+) [Pubmed|23416295]

Structure

• [PDB|3FEF]

Expression and Regulation

Operons

• genes: [gene|18B09A645AC8E13A1AD3B73A0C828E5A0F5D42BB|lplD]-[gene|EBD0F966FD7B3662814D530064BD307561A5CB15|yetF]
  description: [pubmed|22383849]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|26577401], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

Biological materials

Mutant

• BKE07130 ([gene|18B09A645AC8E13A1AD3B73A0C828E5A0F5D42BB|lplD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATATGAAACACTCCCT, downstream forward: _UP4_TGAGCTTGCCGATTAGCTCT
• BKK07130 ([gene|18B09A645AC8E13A1AD3B73A0C828E5A0F5D42BB|lplD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATATGAAACACTCCCT, downstream forward: _UP4_TGAGCTTGCCGATTAGCTCT

References

• 23416295