kapB

activator of [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB], control of the [SW|phosphorelay]

locus: BSU_31460

pI: 6.82

mw: 14.53 kDa

protein length: 128 aa

gene length: 387 bp

function: control of [SW|sporulation] initiation

product: activator of [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|KinB] in the initiation of [SW|sporulation]

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.2|Proteins controlling the activity of the kinases]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.2|Proteins controlling the activity of the kinases]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,231,399 3,231,785

The protein

Structure

• [PDB|1Y71] (from B. cereus, 61% identity)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|kinB]-[gene|18867F99B039D20DBCF004BC72E35CF0AE8FE467|kapB]
  description: [Pubmed|8497199]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8497199], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [regulon|stringent response|stringent response]: positive regulation, [Pubmed|23378509], in [regulon|stringent response|stringent response]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [pubmed|29321771], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]

  regulation

  • induced upon addition of decoyinine (positive [SW|stringent response]) [Pubmed|23378509]

Biological materials

Mutant

• BKE31460 ([gene|18867F99B039D20DBCF004BC72E35CF0AE8FE467|kapB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTCATCACCTCGCAT, downstream forward: _UP4_TAACCCAGTCTGACAAAAGA
• BKK31460 ([gene|18867F99B039D20DBCF004BC72E35CF0AE8FE467|kapB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTCATCACCTCGCAT, downstream forward: _UP4_TAACCCAGTCTGACAAAAGA

References

• 9426145,12618455,8497199,7592498,29314743