yxnA

general stress protein, similar to ketoacyl reductase, survival of ethanol stress

locus: BSU_40000

pI: 9.31

mw: 39.20 kDa

protein length: 356 aa

gene length: 1071 bp

function: survival of ethanol stress

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 4,108,058 4,109,128

The protein

Protein family

• [SW|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)

Paralogous protein(s)

• [protein|0927C7D45721F47F5ED2646CAAC324A308FD7724|YqjQ]:

Structure

• [PDB|4NBU] ([protein|439B468A13137000FB42E9389391CB4986FFED84|FabG] from Bacillus sp., corresponds to aa 26 ... 255 of YxnA, 32% identity) [pubmed|24212572]

Expression and Regulation

Operons

• genes: [gene|18698DA427051097DEEE63F45F3429B8617B1F48|yxnA]
  description: [Pubmed|10746760]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

Biological materials

Mutant

• MGNA-B762 (yxnA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1761 NBRP B. subtilis, Japan]
• BKE40000 ([gene|18698DA427051097DEEE63F45F3429B8617B1F48|yxnA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGAGAGATCCCTCCTAG, downstream forward: _UP4_TAATCTCTGACCATAAGAAA
• BKK40000 ([gene|18698DA427051097DEEE63F45F3429B8617B1F48|yxnA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGAGAGATCCCTCCTAG, downstream forward: _UP4_TAATCTCTGACCATAAGAAA

References

• 15805528,10746760,24212572