murP

N-acetyl muramic acid permease of the [SW|phosphotransferase systems|phosphotransferase system], EIIBC of the [category|SW 1.2.2|PTS]

locus: BSU_01680

pI: 8.50

mw: 47.44 kDa

protein length: 455 aa

gene length: 1368 bp

function: N-acetyl muramic acid uptake and phosphorylation

product: N-acetyl muramic acid [category|SW 1.2.2|PTS] permease, EIIBC component

essential: no

ec: 2.7.1.69

synonyms: ybbF

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.3|Utilization of cell wall components]
• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.17|Utilization of amino sugars]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.3|Utilization of amino sugars]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue]

Gene

Coordinates: 189,790 191,157

The protein

Catalyzed reaction/ biological activity

• transport and concomitant phosphorylation of N-acetyl muramic acid

Protein family

• [category|SW 1.2.2|PTS] permease, sucrose family [Pubmed|10627040]

Paralogous protein(s)

• [protein|35E6C81BE481C3E57CF47B781580368C3AC15D83|BglP], [protein|531F132F7F6A878F1E1D56977B9898A14272349A|SacX], [protein|AE02C38397AA790E4B216BB6DBABFE907B984D05|SacP], [protein|BC568649A341B2E6341993EDA4BF52BDE18A3294|TreP]

[SW|Domains]

• [SW|PTS EIIB domain] type-1 (aa 8-90) (according to UniProt)
• [SW|PTS EIIC domain] type-1 (aa 116-455) (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|ybbC]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR]: repression, [pubmed|30038046], in [regulon|125872506C400CB6B3DAEA8B5130B064DFBA4494|MurR regulon]

  regulation

  • expressed in late exponential and early stationary phase [Pubmed|20400549]

Biological materials

Mutant

• MGNA-B950 (ybbF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1949 NBRP B. subtilis, Japan]
• BKE01680 ([gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAACCCCTCCCCTTC, downstream forward: _UP4_TAAATTGTTTCTGTTTTAGG
• BKK01680 ([gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAACCCCTCCCCTTC, downstream forward: _UP4_TAAATTGTTTCTGTTTTAGG

References

• 10627040,15060041,22383849,30038046