mleA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

malic enzyme

locus: BSU23550

pI: 4.90

mw: 45.00 kDa

function: malate utilization

product: NAD-dependent malate dehydrogenase

essential: no

ec: 1.1.1.38

synonyms: yqkJ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources]

This gene is a member of the following [SW|regulons]

• [SW|AnsR regulon], [SW|CcpA regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,451,463 -> 2,452,782

The protein

Catalyzed reaction/ biological activity

• (S)-malate + NAD⁺ = pyruvate + CO₂ + NADH (according to Swiss-Prot) malate --> pyruvate

Protein family

• malic enzymes family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|YtsJ]

Expression and Regulation

Operon

• ''[protein|search|ansA]-[protein|search|ansB]-[protein|search|mleN]-[protein|search|mleA]'' [Pubmed|22383849]
• ''[protein|search|mleN]-[protein|search|mleA]'' [Pubmed|22383849]

[SW|Sigma factor]

• ''[protein|search|ansA]'': [protein|search|SigA] [Pubmed|1711029]
• ''[protein|search|mleN]'': [protein|search|SigA] [Pubmed|22900538]

Regulation

• ''[protein|search|ansA]'': expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
• ''[protein|search|mleN]'': repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]

Regulatory mechanism

• [protein|search|AnsR]: transcription repression [Pubmed|11914346]
• [protein|search|CcpA]: transcription repression [Pubmed|22900538]

Biological materials

Mutant

• GP1136 (cat) available in [SW|Stülke] lab

References

• 16788182,11914346,22383849,23136871