SubtiBank SubtiBank
mleA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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mleA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

malic enzyme
locus
BSU23550
pI
4.90
mw
45.00 kDa
function
malate utilization
product
NAD-dependent malate dehydrogenase
essential
no
ec
1.1.1.38
synonyms
yqkJ

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
  • [SW|Categories] containing this gene/protein

  • [SW|utilization of specific carbon sources]
  • This gene is a member of the following [SW|regulons]

  • [SW|AnsR regulon], [SW|CcpA regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,451,463 -> 2,452,782

    The protein

    Catalyzed reaction/ biological activity

  • (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate --> pyruvate
  • Protein family

  • malic enzymes family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|YtsJ]
  • Expression and Regulation

    Operon

  • ''[protein|search|ansA]-[protein|search|ansB]-[protein|search|mleN]-[protein|search|mleA]'' [Pubmed|22383849]
  • ''[protein|search|mleN]-[protein|search|mleA]'' [Pubmed|22383849]
  • [SW|Sigma factor]

  • ''[protein|search|ansA]'': [protein|search|SigA] [Pubmed|1711029]
  • ''[protein|search|mleN]'': [protein|search|SigA] [Pubmed|22900538]
  • Regulation

  • ''[protein|search|ansA]'': expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
  • ''[protein|search|mleN]'': repressed by glucose ([protein|search|CcpA]) [Pubmed|22900538]
  • Regulatory mechanism

  • [protein|search|AnsR]: transcription repression [Pubmed|11914346]
  • [protein|search|CcpA]: transcription repression [Pubmed|22900538]
  • Biological materials

    Mutant

  • GP1136 (cat) available in [SW|Stülke] lab
  • References

  • 16788182,11914346,22383849,23136871