oxdD

oxalate decarboxylase, inner spore coat protein

locus: BSU_18670

pI: 5.36

mw: 43.39 kDa

protein length: 392 aa

gene length: 1179 bp

function: protection of the spore

product: oxalate decarboxylase

essential: no

ec: 4.1.1.2

synonyms: yoaN

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.5|Class V]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]

Gene

Coordinates: 2,037,601 2,038,779

The protein

Catalyzed reaction/ biological activity

• H⁺ + oxalate --> CO₂ + formate (according to UniProt)

Paralogous protein(s)

• [protein|9793E952C985BA268AC28C907900A03F4E8A3253|OxdC]

Structure

• [PDB|1L3J] ([protein|9793E952C985BA268AC28C907900A03F4E8A3253|OxdC], 59% identity) [pubmed|14871895]

[SW|Localization]

• inner spore coat, localization depends on [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|SafA] [Pubmed|22171814]

Expression and Regulation

Operons

• genes: [gene|1568DD457FDD482B3C14954A95F62BE338CBD15D|oxdD]
  description: [Pubmed|14973022]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,14973022], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: repression, [Pubmed|14973022], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|14973022]

Biological materials

Mutant

• MGNA-A842 (yoaN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/842 NBRP B. subtilis, Japan]
• BKE18670 ([gene|1568DD457FDD482B3C14954A95F62BE338CBD15D|oxdD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCACCTCGTTTTAGAAA, downstream forward: _UP4_TAAAGACGTAACAGATCACA
• BKK18670 ([gene|1568DD457FDD482B3C14954A95F62BE338CBD15D|oxdD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCACCTCGTTTTAGAAA, downstream forward: _UP4_TAAAGACGTAACAGATCACA

References

Reviews

• 20464388,23202530

Original publications

• 11546787,15699190,14973022,20601499,22171814,14871895,28870294