leuA

2-isopropylmalate synthase

Locus: BSU_28280

Isoelectric point: 5.66

Molecular weight: 56.74 kDa

Protein length: 518 aa

Gene length: 1557 bp

Function: biosynthesis of leucine

Product: 2-isopropylmalate synthase

Essential: no

E.C.: 2.3.3.13

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of branched-chain amino acids
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,892,138 2,893,694

The protein

Catalyzed reaction/ biological activity

• 3-methyl-2-oxobutanoate + acetyl-CoA + H₂O --> (2S)-2-isopropylmalate + CoA + H⁺ (according to UniProt)

Protein family

• alpha-IPM synthase/homocitrate synthase family (single member, according to UniProt)

Domains

• Pyruvate carboxyltransferase domain (aa 4-266) (according to UniProt)

Structure

• 3EEG (from Cytophaga hutchinsonii atcc 33406, 53% identity, 68% similarity)

Localization

• cytoplasm (according to UniProt)
• membrane PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • T-box: termination/antitermination, via tRNA controlled RNA switch, repression by BCAA, in T-box
  • TnrA: repression, PubMed, in TnrA regulon
  • CodY: repression, PubMed PubMed, in CodY regulon
  • CcpA: activation, PubMed, in CcpA regulon

  Regulation

  • expression is stimulated in the presence of glucose (CcpA) PubMed

  Additional information

  • An antisense RNA is predicted for leuA PubMed
  • the T-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• BKE28280 (leuA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATCACGAAGCGTCGTATCGA, downstream forward: _UP4_TAAAAGAAAGGAGAACGGTT
• BKK28280 (leuA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ATCACGAAGCGTCGTATCGA, downstream forward: _UP4_TAAAAGAAAGGAGAACGGTT

References

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