leuA

2-isopropylmalate synthase

locus: BSU_28280

pI: 5.66

mw: 56.74 kDa

protein length: 518 aa

gene length: 1557 bp

function: biosynthesis of leucine

product: 2-isopropylmalate synthase

essential: no

ec: 2.3.3.13

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,892,138 2,893,694

The protein

Catalyzed reaction/ biological activity

• 3-methyl-2-oxobutanoate + acetyl-CoA + H₂O --> (2S)-2-isopropylmalate + CoA + H⁺ (according to UniProt)

Protein family

• alpha-IPM synthase/homocitrate synthase family (single member, according to UniProt)

[SW|Domains]

• Pyruvate carboxyltransferase domain (aa 4-266) (according to UniProt)

Structure

• [PDB|3EEG] (from ''Cytophaga hutchinsonii atcc 33406'', 53% identity, 68% similarity)

[SW|Localization]

• cytoplasm (according to UniProt)
• membrane [Pubmed|18763711]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|E6EDDB6D1EDA85D73A0B78A656511D38346A625B|ilvB]-[gene|49E2D2EE480C2FFDED0C35E3AF0DBED5AD819B5F|ilvH]-[gene|EC1CB2FD563EFDA391D13EB8ADFDC8C9734E9D57|ilvC]-[gene|1502AED337F59DFFCEAFF68FE111C5AA6155701B|leuA]-[gene|105E3452D7F142A7D7616E35AF3FB753C9B63E38|leuB]-[gene|AC459429A9C50463FD947C1CF9EA919B6FE3B335|leuC]-[gene|0EB4C6EE398B14B9284FFAEDBC26C866CE2070D7|leuD]
  description: [Pubmed|1577690]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1577690], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|T-box|T-box]: termination/antitermination, via tRNA controlled [SW|RNA switch], repression by BCAA, in [regulon|T-box|T-box]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|15547269], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [pubmed|12618455] [pubmed|18083814], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: activation, [Pubmed|12193635], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expression is stimulated in the presence of glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [PubMed|12193635]

  additional information

  • An [SW|ncRNA|antisense RNA] is predicted for [gene|1502AED337F59DFFCEAFF68FE111C5AA6155701B|leuA] [PubMed|20525796]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• BKE28280 ([gene|1502AED337F59DFFCEAFF68FE111C5AA6155701B|leuA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCACGAAGCGTCGTATCGA, downstream forward: _UP4_TAAAAGAAAGGAGAACGGTT
• BKK28280 ([gene|1502AED337F59DFFCEAFF68FE111C5AA6155701B|leuA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCACGAAGCGTCGTATCGA, downstream forward: _UP4_TAAAAGAAAGGAGAACGGTT

References

• 15060025,12193635,19258532,8289305,18641142,20525796,15547269,12618455,12107147,18763711,25157083,20935095,24163341,15378759,25755103,26220295