ptsI [Thu Jan 14 2016 18:53:13 GMT+0100 (CET)]

Enzyme I, general (non sugar-specific) component of the HPr)

locus: BSU13910

pI: 4.00

mw: 62.00 kDa

function: PTS-dependent sugar transport

product: phosphotransferase system (PTS) enzyme I

essential: no

ec: 2.7.3.9

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.1|General PTS proteins]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

[SW|Categories] containing this gene/protein

• [SW|phosphotransferase systems], [SW|phosphoproteins], [SW|most abundant proteins]

This gene is a member of the following [SW|regulons]

• [SW|GlcT regulon], [SW|stringent response]

Gene

Coordinates on the chromosome (coding sequence): 1,459,650 -> 1,461,362

The protein

Catalyzed reaction/ biological activity

• Phosphoenolpyruvate protein L-histidine = pyruvate protein N(pi)-phospho-L-histidine (according to Swiss-Prot) PEP-dependent autophosphorylation on His-189, transfer of the phosphoryl group to [PtsH|search|HPr] (His-15)

Protein family

• PEP-utilizing enzyme family (according to Swiss-Prot) PEP-utilizing enzyme family

[SW|Domains]

• HPr binding site (N-Terminal Domain)
• pyruvate binding site (C-Terminal Domain)
• pyrophosphate/phosphate carrier histidine (central Domain)

Modification

• transient autophosphorylation on His-189
• ''in vivo'' also phosphorylated on Ser-34 or Ser-36 [Pubmed|17218307]

[SW|Cofactors]

• Magnesium

Structure

• [http://www.proteopedia.org/wiki/index.php/2wqd 2WQD] (Enzyme I from ''Staphylococcus aureus'') [Pubmed|19801641]

[SW|Localization]

• cytoplasm, even distribution [Pubmed|23475962]

[SW|Interactions]

• [protein|search|PtsI]-[protein|search|PtsH]

Expression and Regulation

Operon

• ''[protein|search|ptsG]-[protein|search|ptsH]-[protein|search|ptsI]'' [Pubmed|11902727]
• ''[protein|search|ptsH]-[protein|search|ptsI]'' [Pubmed|11902727]

[SW|Sigma factor]

• ''[protein|search|ptsG]'': [protein|search|SigA] [Pubmed|11902727]
• ''[protein|search|ptsH]'': [protein|search|SigA] [Pubmed|11902727]

Regulation

• expression activated by glucose (2 fold) ([protein|search|GlcT]) [Pubmed|12850135]
• the ''[protein|search|ptsH]'' promoter is constitutive [Pubmed|11902727]
• subject to negative stringent control upon amino acid limitation (due to control of'' [protein|search|ptsG]'' transcription initiation) [Pubmed|20081037]

Regulatory mechanism

• ''[protein|search|ptsG]'': transcriptional antitermination via the [protein|search|GlcT]-dependent [SW|RNA switch] [Pubmed|9765562]

Additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Biological materials

Mutant

• available in [SW|Jörg Stülke]'s lab:
• GP864 (ermC)
• GP778 (replacement of ''[protein|search|glcT]'' and the ''[protein|search|ptsG]-[protein|search|ptsH]-[protein|search|ptsI]'' operon by a spc cassette), [Pubmed|22722928]

Expression vector

• pAG3 (His-tag) [Pubmed|9237995], available in [SW|Galinier] lab
• for expression, purification in ''E. coli'' (His-tag), in [SW|pWH844]: pGP813 available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Josef Deutscher], Paris-Grignon, France
• [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]

References

• 12850135,17218307,9721285,20081037,19801641,23475962,24129255,15378759