SubtiBank SubtiBank
hisA
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hisA

phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF
locus
BSU34880
pI
4.84
mw
26.38 kDa
protein length
245 aa Sequence Blast
gene length
738 bp Sequence Blast
function
biosynthesis of histidine
product
phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF
essential
no
ec
5.3.1.16
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.14|Biosynthesis/ acquisition of histidine]
  • Gene

    Coordinates
    3,584,317 3,585,054

    The protein

    Catalyzed reaction/ biological activity

  • 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide (according to Swiss-Prot)
  • Protein family

  • hisA/hisF family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|HisF]
  • Structure

  • [PDB|2VEP] (from ''Streptomyces coelicolor'', 41% identity, 58% similarity) [Pubmed|17967415]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|E8FD92F48BE16F87D7481262F4E49003A78DE78F|hisZ]-[gene|183C8B4F28E601D670B3D997D333AF2B5C4A4759|hisG]-[gene|03274B80B2DFE0A2939981B0E0CB8C34A9B0B102|hisD]-[gene|88ED20DFEBD5D7B985F99D4494142FBA699A1E91|hisB]-[gene|DEB3122A3EA36545759FAA6F86F71F971CA5F011|hisH]-[gene|148F0C7983250F50676D234DB713E532AA55DDBF|hisA]-[gene|C22C15754859B2510E52622E6A5DFAF97A7AD4E5|hisF]-[gene|5C84CA2A9E22173E144F519389F545F107A267D9|hisI]
    description
    [Pubmed|12107147]

    regulatory mechanism

  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR]: regulation, [pubmed|30355672], in [regulon|F4097349A563503468A2A14F062AEAC532C7917A|YlxR regulon]
  • regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
  • expression depends on functional [protein|F4097349A563503468A2A14F062AEAC532C7917A|YlxR] [pubmed|30355672]
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    Biological materials

    Mutant

  • 1A626 ( ''hisA''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A626&Search=1A626 BGSC]
  • BKE34880 ([gene|148F0C7983250F50676D234DB713E532AA55DDBF|hisA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGCCGGATATAATGTAAAAG, downstream forward: _UP4_GCGCTTGAAAGGGTGAAGCG
  • BKK34880 ([gene|148F0C7983250F50676D234DB713E532AA55DDBF|hisA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGCCGGATATAATGTAAAAG, downstream forward: _UP4_GCGCTTGAAAGGGTGAAGCG
  • References

  • 12107147