SubtiBank SubtiBank
gltX
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

gltX

glutamyl-tRNA synthetase, universally conserved protein
locus
BSU00920
pI
5.02
mw
55.56 kDa
protein length
483 aa Sequence Blast
gene length
1449 bp Sequence Blast
function
translation
product
glutamyl-tRNA synthetase
essential
yes
ec
6.1.1.17
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.10|Aminoacyl-tRNA synthetases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]
  • Gene

    Coordinates
    111,047 → 112,498

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP L-glutamate tRNA(Glu) = AMP diphosphate L-glutamyl-tRNA(Glu) (according to Swiss-Prot)
  • Protein family

  • class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Structure

  • [PDB|3AKZ] (complex with tRNA-Gln from ''Thermotoga maritima'', 38% identity, 62% similarity) [Pubmed|20882017]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • [SW|universally conserved protein]
  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|132E91DB8C81CD8C4F5160B2C068B74DA10AE224|gltX]-[gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]-[gene|E5826A99769E2FE45959A9D623D6467A8DDDEB81|cysS]-[gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]-[gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]-[gene|172652156ABFE72D5BAAC22729CE17CA947538B5|yacP]
    description
    [Pubmed|7510287]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7510287], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|T-box|T-box]: transcription antitermination, overlaps a transcription terminator upstream of [protein|5B9D5DA130DC3654F386684156BDC350DD05DB60|CysE], in [regulon|T-box|T-box]
  • regulation

  • expression transiently increases in the forespore [Pubmed|22848659]
  • view in new tab

    References

  • 12884008,20882017,22848659,15378759