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ligD [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]
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ligD [Tue Dec 13 2016 11:16:32 GMT+0100 (CET)]

DNA repair polymerase/ ligase in non-homologous end joining DNA repair
locus
BSU13400
pI
6.00
mw
70.00 kDa
function
non-homologous end joining DNA repair, repair of gapped DNA substrates
product
DNA repair polymerase/ ligase
essential
no
synonyms
ykoU

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.3|Spore-encoded non-homologous end joining system]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [SW|Categories] containing this gene/protein

  • [SW|DNA repair/ recombination], [SW|sporulation proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigG regulon], [SW|SpoVT regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,404,518 -> 1,406,353

    Phenotypes of a mutant

  • sensitivity to ionizing radiation in the stationary phase [Pubmed|12215643]
  • sensitivity of spores to several DNA-damaging treatments known to cause double strand breaks, such as UV-ray, X-ray, ultrahigh vacuum and wet heat [Pubmed|16497325,17293412]
  • a ''[protein|search|ykoV]-[protein|search|ligD]'' double mutant is sensitive to radiation [Pubmed|24123749]
  • The protein

    Catalyzed reaction/ biological activity

  • has inherent polymerization and ligase activities that allow it to fill the short gaps that can arise after realignment of the broken ends and to seal the resulting nicks, contributing to genome stability during the stationary phase and germination
  • has an intrinsic 5'-2-deoxyribose-5-phosphate (dRP) lyase activity located at the N-terminal ligase domain [Pubmed|26826709]
  • [SW|Domains]

  • N-terminal DNA ligase catalytic domain (aa 1 - 331) linked to a C-terminal polymerase domain (aa 332 - 611) [Pubmed|23691176]
  • [SW|Interactions]

  • [protein|search|YkoV]-[protein|search|LigD], to stimulate [SW|LigD] activity [Pubmed|23691176,26961308]
  • Expression and Regulation

    Operon

  • ''[protein|search|ykoV]-[protein|search|ligD]'' [Pubmed|16497325]
  • [SW|Sigma factor]

  • [protein|search|SigG] [Pubmed|16497325]
  • Regulation

  • expressed during [SW|sporulation] in the forespore ([protein|search|SigG], [SW|SpoVT]) [Pubmed|16497325]
  • Regulatory mechanism

  • [SW|SpoVT]: transcription activation [Pubmed|16497325]
  • Biological materials

    Mutant

  • BP141 (''[protein|search|ykoV]-[protein|search|ligD]''::''kan'') available in [SW|Fabian Commichau]'s lab
  • BP142 (''[protein|search|ykoW]-[protein|search|ykoV]-[protein|search|ligD]''::''kan'') available in [SW|Fabian Commichau]'s lab
  • References

    Reviews

  • 17938628,22933559
  • Original publications

  • 16497325,23691176,12215643,11075926,11566200,17293412,24123749,24123749,25355514,26826709,26961308