metA [2019-07-03 11:52:13]

homoserine O-succinyltransferase

locus: BSU21910

pI: 6.41

mw: 25.85 kDa

protein length: 301 aa

gene length: 906 bp

function: biosynthesis of methionine

product: homoserine O-succinyltransferase

essential: no

ec: 2.3.1.46

synonyms: metB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

Gene

Coordinates: 2,305,378 2,306,283

The protein

Catalyzed reaction/ biological activity

• Succinyl-CoA + L-homoserine = CoA + O-succinyl-L-homoserine (according to Swiss-Prot) O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate (according to Swiss-Prot)

Structure

• [PDB|2GHR] (from ''Bacillus cereus'', 60% identity, 77% similarity) [Pubmed|17546672]

Expression and Regulation

Operons

• genes: [gene|1217330ECD588E24E42FF9FED2A097CEF32785F3|metA]-[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

Biological materials

Mutant

• BKE21910 ([gene|1217330ECD588E24E42FF9FED2A097CEF32785F3|metA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTGCCACCTCCATTAT, downstream forward: _UP4_TAATTTTTCTTTTTTTAGTG
• BKK21910 ([gene|1217330ECD588E24E42FF9FED2A097CEF32785F3|metA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTGCCACCTCCATTAT, downstream forward: _UP4_TAATTTTTCTTTTTTTAGTG

References

• 17546672,18179421,28581482