SubtiBank SubtiBank
metA
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metA

homoserine O-succinyltransferase
locus
BSU21910
pI
6.41
mw
25.85 kDa
protein length
301 aa Sequence Blast
gene length
906 bp Sequence Blast
function
biosynthesis of methionine
product
homoserine O-succinyltransferase
essential
no
ec
2.3.1.46
synonyms
metB

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • Gene

    Coordinates
    2,305,378 2,306,283

    The protein

    Catalyzed reaction/ biological activity

  • Succinyl-CoA + L-homoserine = CoA + O-succinyl-L-homoserine (according to Swiss-Prot) O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate (according to Swiss-Prot)
  • Structure

  • [PDB|2GHR] (from ''Bacillus cereus'', 60% identity, 77% similarity) [Pubmed|17546672]
  • Expression and Regulation

    Operons

    genes
    [gene|1217330ECD588E24E42FF9FED2A097CEF32785F3|metA]-[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
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    Biological materials

    Mutant

  • BKE21910 ([gene|1217330ECD588E24E42FF9FED2A097CEF32785F3|metA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTGCCACCTCCATTAT, downstream forward: _UP4_TAATTTTTCTTTTTTTAGTG
  • BKK21910 ([gene|1217330ECD588E24E42FF9FED2A097CEF32785F3|metA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACGTGCCACCTCCATTAT, downstream forward: _UP4_TAATTTTTCTTTTTTTAGTG
  • References

  • 17546672,18179421,28581482