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argF [Mon Aug 31 2015 13:53:36 GMT+0200 (CEST)]
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argF [Mon Aug 31 2015 13:53:36 GMT+0200 (CEST)]

ornithine carbamoyltransferase
locus
BSU11250
pI
4.97
mw
34.00 kDa
function
biosynthesis of arginine
product
ornithine carbamoyltransferase
essential
no
ec
2.1.3.3
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.4|Biosynthesis/ acquisition of arginine]
  • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids]
  • This gene is a member of the following [SW|regulons]

  • [SW|AhrC regulon], [SW|CodY regulon]
  • The protein

    Catalyzed reaction/ biological activity

  • Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline (according to Swiss-Prot)
  • Protein family

  • ATCase/OTCase family (according to Swiss-Prot)
  • Structure

  • [PDB|2EF0] (from ''Thermus thermophilus hb8'', 48% identity, 63% similarity)
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operon

  • ''[protein|search|argC]-[protein|search|argJ]-[protein|search|argB]-[protein|search|argD]-[protein|search|carA]-[protein|search|carB]-[protein|search|argF]'' [Pubmed|7511775]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|7511775]
  • Regulation

  • repressed by casamino acids ([protein|search|CodY]) [Pubmed|12107147]
  • repressed by arginine ([protein|search|AhrC]) [Pubmed|1312212]
  • Regulatory mechanism

  • [protein|search|AhrC]: transcription repression [Pubmed|1312212]
  • [protein|search|CodY]: transcription repression [Pubmed|24843172]
  • Biological materials

    Mutant

  • 1A605 ( ''argF''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A605&Search=1A605 BGSC]
  • 1A606 ( ''argF''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A606&Search=1A606 BGSC]
  • References

  • 6096675,24843172,12107147