floA [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

flottilin-like protein (in addition to FloT), resistence protein (against sublancin), accessory role in resistance to cefuroxime

locus: BSU25380

pI: 4.00

mw: 35.00 kDa

function: resistance against sublancin

product: flottilin-like protein

essential: no

synonyms: yqfA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|membrane dynamics], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|resistance against toxins/ antibiotics], [SW|membrane proteins]

This gene is a member of the following [SW|regulons]

• [SW|SigW regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,617,449 -> 2,618,444

Phenotypes of a mutant

• reduced [SW|protein secretion] [Pubmed|23651456]
• a ''[protein|search|floT] [protein|search|floA]'' double mutant does not induce [protein|search|KinC]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]
• a ''[protein|search|floT] [protein|search|floA]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]
• a ''[protein|search|floT] [protein|search|floA]'' double mutant has a [SW|sporulation] defect, due to the lack of [protein|search|FtsH] [Pubmed|22882210]
• a ''[protein|search|floA]'' mutant produces increased amount of pulcherriminic acid [Pubmed|25909364]
• a ''[protein|search|floA]'' mutant has reduced sensitivity to vancomycin [Pubmed|25909364]

The protein

Catalyzed reaction/ biological activity

• controls protease activity of [protein|search|FtsH] [Pubmed|24222488]

Protein family

• UPF0365 family (according to Swiss-Prot)

[SW|Localization]

• membrane [Pubmed|18763711]
• co-localizes with [protein|search|FloT] in discrete foci in the membrane [Pubmed|20713508]
• forms focal structure in the cytoplasma membrane [Pubmed|22753055]
• 13 foci [protein|search|FloA] of are detected in strain 3610 [Pubmed|25909364]

[SW|Interactions]

• oligomerization depends on the glutamate-alanine repeats [Pubmed|25909364]
• [protein|search|FloA]-[protein|search|FtsH] [Pubmed|22882210]
• [protein|search|FloT]-[protein|search|FloA] [Pubmed|26297017,23651456]
• [protein|search|FloA]-[protein|search|PhoR] [Pubmed|25909364]

Expression and Regulation

Operon

• ''[protein|search|yqeZ]-[protein|search|floA]-[protein|search|yqfB]'' [Pubmed|11866510]

[SW|Sigma factor]

• [protein|search|SigW] [Pubmed|11866510]

Regulation

• expressed under stress conditions ([protein|search|SigW]) [Pubmed|11866510]

Biological materials

Mutant

• JS119 (3610 markerless), available in [SW|Daniel Lopez]'s lab
• DL1401 (168 ''ΔfloA''::mls), available in [SW|Daniel Lopez]'s lab

GFP fusion

• GK192 (3610 ''lacA''::P''floT-yfp''(mls)), available in [SW|Daniel Lopez]'s lab
• JS136 (3610 ''amyE::floT''-gfp(spc)), available in [SW|Daniel Lopez]'s lab
• JS134 (3610 ''lacA::floA''-mEos2 (mls)), available in [SW|Daniel Lopez]'s lab
• JS167 (3610 ''lacA::floA''-PAmCherry (mls)), available in [SW|Daniel Lopez]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]) for FloA [Pubmed|25909364], available in [SW|Daniel Lopez]'s lab

References

Reviews

• 25652542

Original publications

• 16629676,11866510,18763711,20713508,22753055,22882210,23651456,22178969,24222488,25909364,26297017,27362352