bshC

cysteine-adding enzyme required for the synthesis of bacillithiol

locus: BSU_15120

pI: 5.25

mw: 62.44 kDa

protein length: 539 aa

gene length: 1620 bp

function: biosynthesis of bacillithiol

product: cysteine-adding enzyme

essential: no

synonyms: yllA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.2|Biosynthesis of bacillithiol]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 1,578,376 1,579,995

Phenotypes of a mutant

• impaired growth in minimal medium, reduced activity of enzymes containing FeS cluster [Pubmed|25988368]
• sensitivity to superoxide (paraquate) stress [Pubmed|25988368]

The protein

Protein family

• BshC family (single member, according to UniProt)

Structure

• [PDB|4WBD] [Pubmed|25496067]

Expression and Regulation

Operons

• genes: [gene|0F153803781C9AC8321FB14F926B645C45E53B00|ylbQ]-[gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]
  description: [Pubmed|20308541]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23894131], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|23894131], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

  regulation

  • ''[protein|search|ylbQ]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

Biological materials

Mutant

• MGNA-B128 (bshC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1127 NBRP B. subtilis, Japan]
• a ''bshC'' null mutant is available in [SW|John Helmann]s lab
• BKE15120 ([gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTAGAACTTCCTTT, downstream forward: _UP4_TGAAATAAAGTTTTAAAGAA
• BKK15120 ([gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTAGAACTTCCTTT, downstream forward: _UP4_TGAAATAAAGTTTTAAAGAA
• BP953 ([gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]::cat ([gene|651D1E534490276B9BE7BFE7B5819176AE09891E|pdxS]-[gene|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT])::tet), available in [SW|Fabian Commichau]'s lab, [Pubmed|29027347]

References

Reviews

• 28117687

Original Publications

• 25496067,8636036,20308541,19578333,23894131,25988368,29027347