SubtiBank SubtiBank
abn2 [2017-8-11 16:0:3]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

abn2 [2017-8-11 16:0:3]

endo-1,5-alpha-L-arabinosidase
locus
BSU39330
pI
7.37
mw
52.38 kDa
protein length
469 aa Sequence Blast
gene length
1407 bp Sequence Blast
function
arabinan degradation
product
endo-1,5-alpha-L-arabinosidase
essential
no
synonyms
yxiA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.6|Utilization of arabinan/ arabinose/ arabitol]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    4,039,466 → 4,040,875

    The protein

    Protein family

  • glycosyl hydrolase 43 family (according to Swiss-Prot)

    • Kinetic information

  • With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. [Pubmed|18408032]

    • [SW|Domains]

  • N-terminal catalytic domain with a characteristic β-propeller fold and a C-terminal domain whose function is unknown [Pubmed|24549757]

    • [SW|Cofactors]

  • Ca2+ [Pubmed|24549757]

    • Structure

  • [PDB|2X8F] [Pubmed|20883454]

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862,18408032]

    • Expression and Regulation

    Operons

    genes
    [gene|1130F1D4CBDDF7B8FFCAF929B8BC4D283233679A|abn2]
    description
    [Pubmed|18408032]

    sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|18408032], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, unknown [Pubmed|18408032], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • expression is stimulated by arabinose and pectin and repressed by glucose [Pubmed|18408032]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B716 (yxiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1715 NBRP B. subtilis, Japan]

    • Labs working on this gene/protein

  • [http://www.itqb.unl.pt/research/biology/microbial-genetics [Isabel de Sa-Nogueira]], Lisboa, Portugal [homepage]

    • References

  • 18607095,24549757,18408032,18957862,12850135,18408032,20883454