cwlS [2018-11-07 16:48:53]

D,L-endopeptidase, peptidoglycan hydrolase for cell separation

locus: BSU19410

pI: 10.44

mw: 44.07 kDa

protein length: 414 aa

gene length: 1242 bp

function: cell separation

product: D,L-endopeptidase, peptidoglycan hydrolase

essential: no

synonyms: yojL

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.4|Endopeptidases]

Gene

Coordinates: 2,115,425 → 2,116,669

The protein

Catalyzed reaction/ biological activity

• cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]
• degradation of gamma-polyglutamic acid [pubmed|29458655]

Protein family

• [SW|peptidase C40 family] (according to UniProt)

[SW|Domains]

• contains four N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|24355088,18430080]
• C-terminal D,L-endopeptidase domain ([SW|NlpC/P60 domain]) [pubmed|29458655,22139507]

Effectors of protein activity

• both enzymatic activities are inhibited by interaction with [protein|0CA55371306D4BD768CFA82027DCB4D581BCCB87|IseA] [pubmed|29458655]

Structure

• [PDB|4XCM] (from Thermus thermophilus, 34% identity) [pubmed|25760608]

[SW|Localization]

• cell membrane (according to Swiss-Prot)
• localizes to cell septa and poles [Pubmed|22139507]

Expression and Regulation

Operons

• genes: [gene|0FBE3F7ECACA7FCFAA4EE32E9B2BB35F0B793C0F|cwlS]
  description: [pubmed|22383849]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [pubmed|22139507], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [pubmed|22139507], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [pubmed|], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh]: repression, synergistic transcription repression with [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB] [Pubmed|20817675], in [regulon|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, synergistic transcription repression with [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh] [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed by glucose (3-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|12850135]

• genes: [gene|0FBE3F7ECACA7FCFAA4EE32E9B2BB35F0B793C0F|cwlS]-[gene|3B45734D18D01BAB6D244DE3547C84F9488075FB|yojM]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B424 (yojL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1423 NBRP B. subtilis, Japan]
• BKE19410 (Δ[gene|0FBE3F7ECACA7FCFAA4EE32E9B2BB35F0B793C0F|cwlS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTCAACCTCCTAAA, downstream forward: _UP4_TAAAAATGAGTATGACAAAA
• BKK19410 (Δ[gene|0FBE3F7ECACA7FCFAA4EE32E9B2BB35F0B793C0F|cwlS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTCAACCTCCTAAA, downstream forward: _UP4_TAAAAATGAGTATGACAAAA

References

Reviews

• 18430080,18266855

Original publications

• 16855244,12850135,22139507,20817675,24355088,29458655,29458657,25760608