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ygaD
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ygaD

similar to [SW|ABC transporter] (fused ATP-binding and membrane protein)
locus
BSU_08690
pI
7.03
mw
66.59 kDa
protein length
589 aa Sequence Blast
gene length
1770 bp Sequence Blast
function
unknown
product
[SW|ABC transporter] (fused ATP-binding and membrane protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.6|Exporters of unknown function]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    939,322 941,091

    The protein

    Protein family

  • [SW|ABC transporter superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|YfiB], [protein|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|BmrC], [protein|70D7098BEBD5E53B4B9B8C3394FAAF972C65EE22|YknU], [protein|9B2766D2F8892AA17DB480216570C81C33355C3B|BmrD], [protein|C6B0ACB9D9703DD8141FD7D9D62DB22FB48F458B|YknV], [protein|D5A3C316BD4567A0155F70330E32ACDE18F063FE|YwjA], [protein|E85814EDF232D21A42380D559EAD2CDFB41F19DB|YfiC], [protein|ED1F82463BAA28B67184BFAB5CB23CF26A62999D|BmrA]

    • [SW|Domains]

  • has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]
  • [SW|ABC transmembrane type-1 domain] (aa 19-307) (according to UniProt)
  • [SW|ABC transporter domain] (aa 341-576) (according to UniProt)

    • Structure

  • [PDB|2HYD] (from Staphylococcus aureus, 61% identity) [pubmed|16943773]

    • [SW|Localization]

  • cell membrane [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|FFC7595CD0930089B768BB36816A3FE470501236|ygaC]-[gene|0EB4D3E1B9AF39ECCBD79EB559FAE5BB0EE4C156|ygaD]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A244 (ygaD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/244 NBRP B. subtilis, Japan]
  • BKE08690 ([gene|0EB4D3E1B9AF39ECCBD79EB559FAE5BB0EE4C156|ygaD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08690 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CACGGTTGATTCCCCCTCTC, downstream forward: _UP4_TAAAACATCAATCTTATATC
  • BKK08690 ([gene|0EB4D3E1B9AF39ECCBD79EB559FAE5BB0EE4C156|ygaD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGGTTGATTCCCCCTCTC, downstream forward: _UP4_TAAAACATCAATCTTATATC

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

  • 10092453,16943773