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azoR2
similar to NAD(P)H dehydrogenase (quinone)
function
resistance to 2-methylhydroquinone
Genomic Context
categories
[category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]Gene
Coordinates
3,445,442 3,446,077
The protein
Catalyzed reaction/ biological activity
anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ --> 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H+ + 2 NADH (according to UniProt)Protein family
azoreductase type 1 family (with [protein|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|AzoR1], according to UniProt)Paralogous protein(s)
[protein|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|AzoR1] Structure
[PDB|3P0R] (from B. anthracis, 43% identity, 61% similarity)Biological materials
Mutant
MGNA-A444 (yvaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/444 NBRP B. subtilis, Japan]BKE33540 ([gene|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTACCAGCCCTTTCG, downstream forward: _UP4_TAATGAAAAAGCCTCCCCTTBKK33540 ([gene|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTACCAGCCCTTTCG, downstream forward: _UP4_TAATGAAAAAGCCTCCCCTTReferences
17725564,18208493,16497325,12107147,27965289