azoR2

similar to NAD(P)H dehydrogenase (quinone)

locus: BSU_33540

pI: 5.11

mw: 23.13 kDa

protein length: 211 aa

gene length: 636 bp

function: resistance to 2-methylhydroquinone

product: azoreductase

essential: no

synonyms: yvaB

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 3,445,442 3,446,077

The protein

Catalyzed reaction/ biological activity

• anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD⁺ --> 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H⁺ + 2 NADH (according to UniProt)

Protein family

• azoreductase type 1 family (with [protein|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|AzoR1], according to UniProt)

Paralogous protein(s)

• [protein|CFFFFE96FB7AEEF7190F81332369F0A6834C34A4|AzoR1]

Structure

• [PDB|3P0R] (from B. anthracis, 43% identity, 61% similarity)

Expression and Regulation

Operons

• genes: [gene|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2]
  description: [Pubmed|17725564]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17725564], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|16497325], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|997B828A99D6D8460712C18ED0CE566B285C22DC|MhqR]: repression, [Pubmed|17725564], in [regulon|997B828A99D6D8460712C18ED0CE566B285C22DC|MhqR regulon]

  regulation

  • induced by catechol and 2-methylhydroquinone (2-MHQ) ([protein|search|MhqR]) [Pubmed|17725564]

Biological materials

Mutant

• MGNA-A444 (yvaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/444 NBRP B. subtilis, Japan]
• BKE33540 ([gene|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTACCAGCCCTTTCG, downstream forward: _UP4_TAATGAAAAAGCCTCCCCTT
• BKK33540 ([gene|0E2B1914A040666BAF042C0BE3F468144E1F1E06|azoR2]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTACCAGCCCTTTCG, downstream forward: _UP4_TAATGAAAAAGCCTCCCCTT

References

• 17725564,18208493,16497325,12107147,27965289