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sdhA [Wed Aug 24 2016 13:22:07 GMT+0200 (CEST)]
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sdhA [Wed Aug 24 2016 13:22:07 GMT+0200 (CEST)]

succinate dehydrogenase (flavoprotein subunit)
locus
BSU28440
pI
5.00
mw
65.00 kDa
function
TCA cycle
product
succinate dehydrogenase (flavoprotein subunit)
essential
no
synonyms
citF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|membrane proteins], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|FsrA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,906,335 -> 2,908,095

    The protein

    Catalyzed reaction/ biological activity

  • Succinate acceptor = fumarate reduced acceptor (according to Swiss-Prot)

    • Protein family

  • FRD/SDH subfamily (according to Swiss-Prot)

    • Modification

  • phosphorylated [Pubmed|16493705], [Pubmed|17726680]

    • [SW|Cofactors]

  • Fe

    • Effectors of protein activity

  • Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide [Pubmed|3910107]
  • Activated by Cytochrome b558 [Pubmed|6799760]

    • Structure

  • [PDB|1NEK] (''E. coli'')

    • [SW|Localization]

  • attached to the membrane [Pubmed|18763711]

    • [SW|Interactions]

  • [protein|search|SdhA]-[protein|search|SdhB] (as part of the [protein|search|SdhC]-[protein|search|SdhB]-[protein|search|SdhA] complex)
  • [protein|search|FloT]-[protein|search|SdhA] [Pubmed|23651456]
  • part of a [SW|respiratory supercomplex] [Pubmed|23880299]

    • Additional information

  • This enzyme is a membrane-bound trimer [Pubmed|3910107] [Pubmed|6799760]
  • One subunit is bound to cytochrome b558, and this subunit is the one bound to the cytosolic side of the membrane [Pubmed|3910107] [Pubmed|6799760]
  • Another subunit is the flavoprotein one, required for FAD usage [Pubmed|3910107] [Pubmed|6799760]
  • The other subunit has an iron-sulphur domain necessary for the catalytic activity [Pubmed|3910107] [Pubmed|6799760]
  • extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/Succinate_Dehydrogenase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|sdhC]''-''[protein|search|sdhA]''-''[protein|search|sdhB]'' [Pubmed|3036777]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|2495271]

    • Regulation

  • constitutive
  • part of the iron sparing response ([protein|search|FsrA]) [Pubmed|22389480]

    • Regulatory mechanism

  • [protein|search|FsrA]: translation inhibition [Pubmed|22389480]

    • Biological materials

    Mutant

  • GP743 (''[protein|search|sdhC]-[protein|search|sdhA]'', cat), available in [SW|Jörg Stülke]'s lab
  • GP792 (''[protein|search|sdhC]-[protein|search|sdhA]-[protein|search|sdhB]''::''phleo''), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 11803018

    • Original publications

  • 2987185,1707123,6401289,6406223,1324713,3036777,2495271,3027051,22389480,17726680,18763711,17726680,16493705,3910107,6799760,12560550,23651456,23880299