pchR [2018-04-23 16:06:51]

transcriptional repressor ([SW|MarR family]), controls the expression of genes involved in pulcherriminic acid biosynthesis

locus: BSU35080

pI: 8.09

mw: 19.54 kDa

protein length: 169 aa

gene length: 507 bp

function: control of pulcherriminic acid biosynthesis

product: transcription factor ([SW|MarR family])

essential: no

synonyms: yzhA,yvmB

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]

Gene

Coordinates: 3,604,993 → 3,605,502

Phenotypes of a mutant

• inactivation of ''[gene|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]'' causes widespread disruption of proteins involved in cellular processes that depend on iron availability, with pleiotropic effects on proteins associated with carbon metabolism, translation, stress response, biosynthesis of cell wall, and sporulation initiation [Pubmed|27542896]

The protein

Protein family

• [SW|MarR family] of transcriptional regulators [Pubmed|27542896]

Additional information

• Proposed consensus sequence for DNA-binding site: GTTYMMYMGTAAAC [Pubmed|27542896]

Expression and Regulation

Operons

• genes: [gene|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]-[gene|7F10DEEDEF8B679F4F2C5EAD5752F4E399DB0D57|yvmA]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135]; [protein|0D555F2AB7DC863E6FF388888308E980514DB719|PchR] auto-repression [Pubmed|27542896], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|0D555F2AB7DC863E6FF388888308E980514DB719|PchR]: repression, in [regulon|0D555F2AB7DC863E6FF388888308E980514DB719|PchR regulon]

  regulation

  • induced by iron starvation [Pubmed|27542896]

Biological materials

Mutant

• CS217 (''[gene|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]
• BKE35080 (Δ[gene|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGGCTACCTTCTTTCTT, downstream forward: _UP4_TAAACAAAAAGGCGGTGTAC
• BKK35080 (Δ[gene|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGGCTACCTTCTTTCTT, downstream forward: _UP4_TAAACAAAAAGGCGGTGTAC

Labs working on this gene/protein

• Sandrine Auger, Micalis Institute, INRA, AgroParisTech, France

References

• 12850135,22383849,27542896,27197833