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nahA
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nahA

RNA pyrophosphohydrolase, converts primary transcripts to 5’-monophosphate RNA
Locus
BSU_34780
Isoelectric point
4.99
Molecular weight
18.07 kDa
Protein length
158 aa Sequence Blast
Gene length
477 bp Sequence Blast
Function
initiation of RNA degradation
Product
RNA pyrophosphohydrolase
Essential
no
Synonyms
yvcI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides
  • Information processing → RNA synthesis and degradation → RNases → RNA pyrophosphohydrolase
  • Information processing → Targets of second messengers → Targets of (p)ppGpp

    • Gene

    Coordinates
    3,572,413 3,572,889

    The protein

    Catalyzed reaction/ biological activity

  • converts primary transcripts to 5’-monophospate RNA PubMed
  • prefers G-initiating RNAs and required at least one unpaired nucleotide at the 5’-end of its substrates, with the 5’-terminal nucleotide determining whether primarily ortho- or pyrophosphate is released PubMed

    • Protein family

  • Nudix hydrolase (according to UniProt)

    • Domains

  • Nudix hydrolase domain (aa 3-130) (according to UniProt)

    • Cofactors

  • Mn2+ PubMed

    • Structure

  • 3FK9 (the protein from B. halodurans, 59% identity/ 82% similarity)

    • Expression and Regulation

    Operons

    Genes
    nahA-yvcJ-glmR-whiA-crh-yvcN
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • CcpA: repression, in CcpA regulon

    • Regulation

  • constitutive expression at both protein and RNA levels PubMed

    • Additional information

  • A ncRNA is predicted between 'yvcI' and 'trxB' PubMed
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    Biological materials

    Mutant

  • MGNA-B642 (yvcI::erm), available at the NBRP B. subtilis, Japan
  • BKE34780 (ΔnahA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACCTAACCTTCGTCCTGTC, downstream forward: _UP4_TAGAAAGACAAGTCAGGGGG
  • BKK34780 (ΔnahA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACCTAACCTTCGTCCTGTC, downstream forward: _UP4_TAGAAAGACAAGTCAGGGGG

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • References

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