pcrB

heptaprenylglyceryl phosphate synthase

locus: BSU_06600

pI: 4.20

mw: 24.94 kDa

protein length: 228 aa

gene length: 687 bp

function: unknown

product: heptaprenylglyceryl phosphate synthase

essential: no

synonyms: yerE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]

Gene

Coordinates: 718,622 719,308

The protein

Catalyzed reaction/ biological activity

• production of heptaprenylglyceryl phosphate from heptaprenyl diphosphate and glycerol-1-phosphate [Pubmed|23322418]
• all-trans-heptaprenyl diphosphate + sn-glycerol 1-phosphate --> 3-heptaprenyl-sn-glycero-1-phosphate + diphosphate (according to UniProt)

Protein family

• GGGP/HepGP synthase family (single member, according to UniProt)

Structure

• [PDB|1VIZ] [Pubmed|23322418]

Expression and Regulation

Operons

• genes: [gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]-[gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA]-[gene|03EFF93C1B0CE075AE068ECC4EDE9BE5D03C857E|ligA]-[gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]
  description: [Pubmed|9701819]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]

Biological materials

Mutant

• MGNA-A926 (yerE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/926 NBRP B. subtilis, Japan]
• BKE06600 ([gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCATACGTTCCTCCAT, downstream forward: _UP4_TAGTAAATTGTACGGATTTG
• BKK06600 ([gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCATACGTTCCTCCAT, downstream forward: _UP4_TAGTAAATTGTACGGATTTG

References

• 9701819,22614947,21761520,18558723,23322418,24684232