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aprE [2017-05-24 12:27:32]
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aprE [2017-05-24 12:27:32]

major extracellular alkaline protease
locus
BSU10300
pI
9.34
mw
39.37 kDa
protein length
381 aa Sequence Blast
gene length
1143 bp Sequence Blast
function
protein degradation
product
extracellular alkaline serine protease (subtilisin E))
essential
no
synonyms
sprE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.2|Extracellular feeding proteases]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    1,104,423 → 1,105,568

    The protein

    Catalyzed reaction/ biological activity

  • Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1 (according to Swiss-Prot)

    • Protein family

  • peptidase S8 family (according to Swiss-Prot)

    • Structure

  • [PDB|1SBC]

    • [SW|Localization]

  • secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operons

    genes
    [gene|0B98DE9CE2D98FFDE9F6EEB4E94FA1E5204BD48D|aprE]
    description
    [pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2447063], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|1898931], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|1906467], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|2504584], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|12950930,15598897,2447063], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|26728191], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

    • regulation

  • repressed by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] [Pubmed|1898931]
  • induced in the presence of external [protein|60D6EB02923D9ADE88F61A8CBBD882BA8BDD457E|ComX] [pubmed|29449835]

    • additional information

  • in a triple ''[SW|abrB] [SW|codY] [SW|scoC]'' mutant, expression occurs during exponential growth [Pubmed|26728191]
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    Biological materials

    Mutant

  • BKE10300 (''ΔaprE::erm trpC2''), available at BGSC
  • KO7 (''ΔnprE ΔaprE Δepr Δmpr ΔnprB Δvpr Δbpr''), available as BGSC 1A1133

    • Antibody

  • **

    • References

    Reviews

  • 20735481

    • Original publications

  • 19710937,3082852,2447063,2447062,19251843,18414485,6322190,12884008,15126467,1898931,1906467,2504584,18957862,11101663,12055299,3131303,1906467,15598897,11325965,14688235,21099137,22745669,23563549,23563567,24115457,25315053,12950930,26728191