major extracellular alkaline protease
function
protein degradation
product
extracellular alkaline serine protease (subtilisin E))
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins][category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.2|Extracellular feeding proteases][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins][SW|Categories] containing this gene/protein
[SW|utilization of nitrogen sources other than amino acids], [SW|proteolysis]This gene is a member of the following [SW|regulons]
[SW|AbrB regulon], [SW|CodY regulon], [SW|DegU regulon], [SW|ScoC regulon], [SW|SinR regulon]Gene
Coordinates on the chromosome (coding sequence)
1,104,423 -> 1,105,568
The protein
Catalyzed reaction/ biological activity
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1 (according to Swiss-Prot)Protein family
peptidase S8 family (according to Swiss-Prot)Structure
[PDB|1SBC][SW|Localization]
secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862][SW|Interactions]
[protein|search|AprE]-[protein|search|PaiA] [Pubmed|11325965]Expression and Regulation
Operon
aprE (according to [http://dbtbs.hgc.jp/COG/prom/aprE.html DBTBS])[SW|Sigma factor]
[protein|search|SigA] [Pubmed|2447063]Regulation
repressed by [protein|search|SinR] [Pubmed|1898931]repressed during exponential growth ([protein|search|ScoC], [protein|search|AbrB]) [Pubmed|1906467,2504584]Regulatory mechanism
[protein|search|SinR]: transcription repression [Pubmed|1898931][protein|search|ScoC]: transcription repression [Pubmed|1906467][protein|search|AbrB]: transcription repression [Pubmed|2504584][protein|search|DegU]-P: transcription activation [Pubmed|12950930,15598897,2447063][SW|CodY]: transcription repression [Pubmed|26728191]Additional information
in a triple ''[SW|abrB] [SW|codY] [SW|scoC]'' mutant, expression occurs during exponential growth [Pubmed|26728191]the mRNA is extremely stable (more than 25 min) [Pubmed|11101663 ]Biological materials
References
Reviews
Original publications
19710937,3082852,2447063,2447062,19251843,18414485,6322190,12884008,15126467,1898931,1906467,2504584,18957862,11101663,12055299,3131303,1906467,15598897,11325965,14688235,21099137,22745669,23563549,23563567,24115457,25315053,12950930,26728191 
