yloS

thiamine pyrophosphokinase

locus: BSU_15800

pI: 5.22

mw: 23.95 kDa

protein length: 214 aa

gene length: 645 bp

function: thiamine salvage

product: thiamine pyrophosphokinase

essential: no

ec: 2.7.6.2

synonyms: thiN

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]

Gene

Coordinates: 1,654,730 1,655,374

The protein

Catalyzed reaction/ biological activity

• ATP + thiamine --> AMP + H⁺ + thiamine diphosphate (according to UniProt)

Protein family

• thiamine pyrophosphokinase family (single member, according to UniProt)

Structure

• [PDB|3LM8]

Expression and Regulation

Operons

• genes: [gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]-[gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]-[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]-[gene|8103B8539338B77CF51B16B7FA76783ED2319F13|rpe]-[gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B136 (yloS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1135 NBRP B. subtilis, Japan]
• BKE15800 ([gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTGATTCCTTTCTCT, downstream forward: _UP4_TAAGCATGTGCTTTCATTCA
• BKK15800 ([gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTGATTCCTTTCTCT, downstream forward: _UP4_TAAGCATGTGCTTTCATTCA

References

Reviews

• 19348578,28031352

Original publications

• 15150256,16291685