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miaA [2017-8-11 16:0:3]
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miaA [2017-8-11 16:0:3]

tRNA isopentenylpyrophosphate transferase
locus
BSU17330
pI
6.00
mw
35.52 kDa
protein length
314 aa Sequence Blast
gene length
942 bp Sequence Blast
function
tRNA modification
product
tRNA isopentenylpyrophosphate transferase
essential
no
ec
2.5.1.8
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • Gene

    Coordinates
    1,866,389 → 1,867,333

    Phenotypes of a mutant

  • inactivation of ''[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]'' reduces sporulation efficiency to 16% that of wild type cells [Pubmed|26735940]
  • The protein

    Catalyzed reaction/ biological activity

  • Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine (according to Swiss-Prot)
  • Protein family

  • IPP transferase family (according to Swiss-Prot)
  • Structure

  • [PDB|2QGN] (from ''Bacillus halodurans'', 57% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
    description
    [pubmed|22383849]
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    genes
    [gene|61B511C49912F1380AEE18AD300255A435F56F94|ymaF]-[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
    description
    [pubmed|22383849]

    regulation

  • expressed during [SW|sporulation ][Pubmed|22383849]
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    References

    The corresponding gene/ protein in other bacteria

  • 11160115,3045085,9294434,26735940