SubtiBank SubtiBank
ispH
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ispH

(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 7th step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_25160
pI
5.61
mw
34.77 kDa
protein length
314 aa Sequence Blast
gene length
945 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase
essential
yes
ec
1.17.1.2
synonyms
yqfP

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,596,535 2,597,479

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] --> (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + 2 reduced [2Fe-2S]-[ferredoxin] (according to UniProt)
  • dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] --> (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H+ + 2 reduced [2Fe-2S]-[ferredoxin] (according to UniProt)
  • Protein family

  • ispH family (single member, according to UniProt)
  • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]
  • Structure

  • [PDB|3F7T] (IspH from ''E. coli'', 38% identity) [Pubmed|19569147]
  • Expression and Regulation

    Operons

    genes
    [gene|9B1EA9872500280D6360EECD3D7344A1974910CA|yqfQ]-[gene|08F1D9AD2D51D8513AFFA57E8ED69DB8EF97AD88|ispH]
    description
    [Pubmed|22383849]
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    genes
    [gene|08F1D9AD2D51D8513AFFA57E8ED69DB8EF97AD88|ispH]
    description
    [Pubmed|22383849]
    view in new tab

    References

  • 17458547,19569147,23840410,27791134