SubtiBank SubtiBank
ispH
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

ispH

(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 7th step in the MEP pathway of isoprenoid biosynthesis
locus
BSU25160
pI
5.61
mw
34.77 kDa
protein length
314 aa Sequence Blast
gene length
942 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase
essential
yes
ec
1.17.1.2
synonyms
yqfP

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,596,535 → 2,597,479

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Isopentenyl diphosphate NAD(P) H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate NAD(P)H (according to Swiss-Prot)
  • Protein family

  • ispH family (according to Swiss-Prot)
  • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]
  • Structure

  • [PDB|3F7T] (IspH from ''E. coli'', 38% identity) [Pubmed|19569147]
  • References

  • 17458547,19569147,23840410,27791134