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comK [Thu Dec 08 2016 16:04:54 GMT+0100 (CET)]
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comK [Thu Dec 08 2016 16:04:54 GMT+0100 (CET)]

competence transcription factor (CTF)
locus
BSU10420
pI
7.00
mw
22.00 kDa
function
regulation of competence and DNA uptake
product
competence transcription factor (CTF)
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [SW|Categories] containing this gene/protein

  • [SW|genetic competence], [SW|transcription factors and their control], [SW|phosphoproteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|CodY regulon], [SW|ComK regulon], [SW|DegU regulon], [SW|Rok regulon], [SW|Spo0A regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,117,109 -> 1,117,687

    Phenotypes of a mutant

  • no expression of competence genes, loss of [SW|genetic competence] [Pubmed|7783616]
  • The protein

    Modification

  • phosphorylated on Arg-65, Arg-157, Arg-161, Arg-165, Arg-186, and Arg-191 [Pubmed|22517742]
  • [SW|Interactions]

  • [protein|search|MecA]-[protein|search|ComK] [Pubmed|10447896]
  • Expression and Regulation

    Operon

  • ''comK'' [Pubmed|8196543]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8196543]
  • Regulation

  • ''[SW|comK]'' has a bistable expression pattern [Pubmed|26110430]
  • repressed by casamino acids [Pubmed|12107147]
  • repressed by [protein|search|Rok] [Pubmed|11849533]
  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|8830686]
  • repressed during logarithmic growth ([protein|search|AbrB]) [Pubmed|12586407]
  • induced at the entry to stationary phase ([SW|Spo0A]-P) [Pubmed|22412392]
  • Regulatory mechanism

  • [protein|search|Rok]: transcription repression [Pubmed|11849533]
  • [protein|search|CodY]: transcription repression [Pubmed|8830686]
  • [protein|search|AbrB]: transcription repression [Pubmed|12586407]
  • [protein|search|ComK]: transcription activation (autoregulation) [Pubmed|8083168]
  • [protein|search|DegU] (non-phosphorylated form): transcription activation [Pubmed|8083168]
  • [SW|Spo0A]-P: transcription activation, acts as antagonist of [protein|search|Rok] [Pubmed|22412392]
  • Additional information

  • degraded by [protein|search|ClpC]-[protein|search|ClpP] (with [protein|search|MecA] as adaptor protein) [PubMed|9890793]
  • half-life of the ''[SW|comK]'' mRNA: 3.9 min, stabilized to 5.4 min in a ''[SW|kre]'' mutant [Pubmed|26110430]
  • Biological materials

    Mutant

  • 1A871 (no resistance), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A871&Search=1A871 BGSC]
  • Expression vector

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • Labs working on this gene/protein

  • [SW|Oscar Kuipers], University of Groningen, The Netherlands
  • [http://molgen.biol.rug.nl/molgen/index.php Homepage]
  • References

    Reviews

  • 19995980,12576575,26110607
  • The [SW|ComK regulon]

  • 11948146,11918817
  • Original Publications

  • 8878039,11849533,17569828,9335269,17560370,12586407,11814663,9108277,9890793,9585513,15819619,10908654,9000055,7746142,8830686,10447896,12028382,8016067,10361283,11703662,12107147,17581123,8016066,17493123,16391071,16436435,12270822,15598897,20300532,12761164,12036071,21085679,22517742,7783616,22412392,8083168,24838881,8196543,26110430,27045827