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sigM [Wed Jun 29 2016 12:57:32 GMT+0200 (CEST)]
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sigM [Wed Jun 29 2016 12:57:32 GMT+0200 (CEST)]

RNA polymerase ECF-type sigma factor SigM, required for adaptation to inhibitors of peptidoglycan synthesis
locus
BSU09520
pI
6.00
mw
19.00 kDa
function
adaptation to inhibitors of peptidoglycan synthesis
product
RNA polymerase ECF-type sigma factor SigM
essential
no
synonyms
yhdM

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [SW|Categories] containing this gene/protein

  • [SW|transcription], [SW|sigma factors and their control], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|resistance against toxins/ antibiotics]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigM regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,029,577 -> 1,030,068

    Phenotypes of a mutant

  • SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl [Pubmed|10216858]
  • ''sigM'' mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts [Pubmed|10216858]
  • increased sensitivity towards beta-lactam antibiotics [Pubmed|22211522]
  • a ''[protein|search|sigM] [protein|search|murJ]'' double mutant is not viable (due to the lack of both lipid II flippases) [Pubmed|25918422]
  • The protein

    Protein family

  • sigma-70 factor family, ECF subfamily (according to Swiss-Prot)
  • Effectors of protein activity

  • expression of [protein|search|CsbB] in the absence of [protein|search|YfhO] causes constitutive activation of [protein|search|SigM] [Pubmed|23632331]
  • shortage of available bactoprenol causes [protein|search|SigM] activation [Pubmed|23632331]
  • [SW|Interactions]

  • [protein|search|SigM]-[protein|search|YhdK]-[protein|search|YhdL] [Pubmed|14993308]
  • [protein|search|SigM]-[protein|search|RpoB]-[protein|search|RpoC]
  • Additional information

  • Expression of the [SW|SigM regulon] in increased in ''[protein|search|ugtP]'' mutants [Pubmed|22362028]
  • Expression and Regulation

    Operon

  • ''[protein|search|sigM]-[protein|search|yhdL]-[protein|search|yhdK]'' [Pubmed|9573210]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|9573210], [protein|search|SigM] [Pubmed|9573210,18179421]
  • Regulation

  • induced by paraquat ([protein|search|SigM]) [Pubmed|12775685]
  • Additional information

  • expression of [protein|search|CsbB] in the absence of [protein|search|YfhO] causes constitutive activation of [protein|search|SigM] [PubMed|23632331]
  • shortage of available bactoprenol causes [protein|search|SigM] activation [PubMed|23632331]
  • Biological materials

    Mutant

  • 1A906 (''sigM''::''kan''), [Pubmed|12207695], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A906&Search=1A906 BGSC]
  • BP129 (''sigM::aphA3''), available in [SW|Fabian Commichau]'s lab
  • Labs working on this gene/protein

  • [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
  • References

    Reviews

  • 24921931,26901131,27344142
  • The [SW|SigM regulon]

  • 18179421,17675383,17434969
  • Other original publications

  • 22362028,18394148,14993308,12399481,12775685,12207695,18820022,15838020,19047346,14651641,16151214,19465659,19745567,10216858,20057163,23103977,23632331,23070162,22211522,20817771,25918422,26399770