RNA polymerase ECF-type sigma factor SigM, required for adaptation to inhibitors of peptidoglycan synthesis
function
adaptation to inhibitors of peptidoglycan synthesis
product
RNA polymerase ECF-type sigma factor SigM
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)][SW|Categories] containing this gene/protein
[SW|transcription], [SW|sigma factors and their control], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|resistance against toxins/ antibiotics]This gene is a member of the following [SW|regulons]
[SW|SigM regulon]Gene
Coordinates on the chromosome (coding sequence)
1,029,577 -> 1,030,068
Phenotypes of a mutant
SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl [Pubmed|10216858]''sigM'' mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts [Pubmed|10216858]increased sensitivity towards beta-lactam antibiotics [Pubmed|22211522]a ''[protein|search|sigM] [protein|search|murJ]'' double mutant is not viable (due to the lack of both lipid II flippases) [Pubmed|25918422]The protein
Protein family
sigma-70 factor family, ECF subfamily (according to Swiss-Prot)Effectors of protein activity
expression of [protein|search|CsbB] in the absence of [protein|search|YfhO] causes constitutive activation of [protein|search|SigM] [Pubmed|23632331]shortage of available bactoprenol causes [protein|search|SigM] activation [Pubmed|23632331][SW|Interactions]
[protein|search|SigM]-[protein|search|YhdK]-[protein|search|YhdL] [Pubmed|14993308][protein|search|SigM]-[protein|search|RpoB]-[protein|search|RpoC]Additional information
Expression of the [SW|SigM regulon] in increased in ''[protein|search|ugtP]'' mutants [Pubmed|22362028]Expression and Regulation
Operon
''[protein|search|sigM]-[protein|search|yhdL]-[protein|search|yhdK]'' [Pubmed|9573210][SW|Sigma factor]
[protein|search|SigA] [Pubmed|9573210], [protein|search|SigM] [Pubmed|9573210,18179421]Regulation
induced by paraquat ([protein|search|SigM]) [Pubmed|12775685]Additional information
expression of [protein|search|CsbB] in the absence of [protein|search|YfhO] causes constitutive activation of [protein|search|SigM] [PubMed|23632331]shortage of available bactoprenol causes [protein|search|SigM] activation [PubMed|23632331]Biological materials
Mutant
1A906 (''sigM''::''kan''), [Pubmed|12207695], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A906&Search=1A906 BGSC]BP129 (''sigM::aphA3''), available in [SW|Fabian Commichau]'s labLabs working on this gene/protein
[SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]References
Reviews
The [SW|SigM regulon]
18179421,17675383,17434969 Other original publications
22362028,18394148,14993308,12399481,12775685,12207695,18820022,15838020,19047346,14651641,16151214,19465659,19745567,10216858,20057163,23103977,23632331,23070162,22211522,20817771,25918422,26399770 
