lnrM

similar to ABC transporter (membrane protein), involved in resistance to linearmycin

Locus: BSU_08320

Isoelectric point: 9.62

Molecular weight: 42.95 kDa

Protein length: 396 aa

Gene length: 1191 bp

Function: resistance to linearmycin

Product: ABC transporter (membrane protein)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Exporters → Efflux of antibiotics
• Groups of genes → Membrane proteins

Gene

Coordinates: 906,764 907,954

Phenotypes of a mutant

• overexpression of lnrM and lnrN stimulates biofilm formation PubMed

The protein

Protein family

• ABC-2 integral membrane protein family (according to UniProt)

Domains

• ABC transmembrane type-2 domain (aa 161-396) (according to UniProt)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: lnrL-lnrM-lnrN
  Description: PubMed

  Sigma factors

  • SigF: sigma factor, in SigF regulon
  • SigG: sigma factor, in SigG regulon

  Regulatory mechanism

  • LnrK: activation, PubMed, in LnrK regulon

  Regulation

  • expression during spore germination is increased under conditions of osmotic stress PubMed
  • induced in the presence of linearmycin and other polyenes (LnrK) PubMed

Biological materials

Mutant

• MGNA-C302 (yfiM::erm), available at the NBRP B. subtilis, Japan
• BKE08320 (lnrM::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCTTTTTTTCATTGCCGTCC, downstream forward: _UP4_CATACAAGATAAGGAGGTAC
• BKK08320 (lnrM::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCTTTTTTTCATTGCCGTCC, downstream forward: _UP4_CATACAAGATAAGGAGGTAC

References

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