fadA

acetyl-CoA C-acyltransferase

locus: BSU_32830

pI: 5.18

mw: 40.96 kDa

protein length: 391 aa

gene length: 1176 bp

function: fatty acid degradation

product: acetyl-CoA C-acyltransferase

essential: no

ec: 2.3.1.16

synonyms: yusK

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

Gene

Coordinates: 3,368,839 3,370,014

The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + acyl-CoA --> 3-oxoacyl-CoA + CoA (according to UniProt)

Protein family

• [SW|thiolase-like superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|226100C0AC13BB345DB0F0F309DEB7F91F3770B0|MmgA], [protein|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|YhfS]

Structure

• [PDB|3SS6] (the protein of ''B. anthracis'', 41% identity, 73% similarity)

Expression and Regulation

Operons

• genes: [gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]-[gene|07498A0A3CB598A6E388540124A4F56E781C86FA|fadA]-[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]
  description: [Pubmed|17189250]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17189250], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR]: activation, [Pubmed|12817086], in [regulon|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR regulon]
  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|21398533], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • ''[protein|4787161A022E938A6D58A505D92369F889C7DE04|FadN]'': induced by long-chain fatty acids ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]) [Pubmed|17189250]
  • expression of the operon is induced during [SW|biofilm formation] [pubmed|31113899]

• genes: [gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]-[gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]-[gene|07498A0A3CB598A6E388540124A4F56E781C86FA|fadA]-[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]
  description: [Pubmed|17189250]

  regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]
  • [protein|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR]: activation, [Pubmed|12817086], in [regulon|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR regulon]

  regulation

  • ''[protein|search|fadM]'': induced by long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]

Biological materials

Mutant

• MGNA-A603 (yusK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/603 NBRP B. subtilis, Japan]
• BKE32830 ([gene|07498A0A3CB598A6E388540124A4F56E781C86FA|fadA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACTGCCTCTTTCATAAGCT, downstream forward: _UP4_TTATGCTAAAGGGGGAAAAA
• BKK32830 ([gene|07498A0A3CB598A6E388540124A4F56E781C86FA|fadA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACTGCCTCTTTCATAAGCT, downstream forward: _UP4_TTATGCTAAAGGGGGAAAAA

References

• 17189250,12817086,17919287