SubtiBank SubtiBank
yhcT
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yhcT

similar to pseudouridylate synthase
locus
BSU_09210
pI
9.64
mw
33.59 kDa
protein length
302 aa Sequence Blast
gene length
909 bp Sequence Blast
function
RNA modification
product
putative [SW|sporulation]-specific pseudouridylate synthase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.3|rRNA modification and maturation/ based on similarity]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

    • Gene

    Coordinates
    995,624 996,532

    The protein

    Catalyzed reaction/ biological activity

  • uridine in RNA --> ψ-uridine in RNA (according to UniProt)

    • Protein family

  • [SW|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
  • pseudouridine synthase RluA family (with [protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|YjbO] and [protein|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|YlyB], according to UniProt)

    • Paralogous protein(s)

  • [protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|YjbO], [protein|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|YlyB]

    • [SW|Domains]

  • [SW|S4 RNA-binding domain] (aa 19-90) (according to UniProt)

    • Structure

  • [PDB|1V9F] (RluD from E. coli, 36% identical) [pubmed|15078091]

    • Expression and Regulation

    Operons

    genes
    [gene|964F04481DFA789CB0F7387B066BE2E2CF88D8E5|S319]-[gene|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A666 (yhcT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/666 NBRP B. subtilis, Japan]
  • BKE09210 ([gene|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTAAATGGCTCCTTTT, downstream forward: _UP4_TGAAGAAAAGCCGCAGGCAC
  • BKK09210 ([gene|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTAAATGGCTCCTTTT, downstream forward: _UP4_TGAAGAAAAGCCGCAGGCAC

    • References

    Research papers

  • 15078091