SubtiBank SubtiBank
bglS [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

bglS [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

endo-beta-1,3-1,4 glucanase
locus
BSU39070
pI
6.48
mw
27.00 kDa
function
lichenan degradation
product
endo-beta-1,3-1,4 glucanase
essential
no
ec
3.2.1.73
synonyms
bgl, licS

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • syns
    bgl, licS

    [SW|Categories] containing this gene/protein

  • [SW|utilization of specific carbon sources], [SW|sporulation proteins]
  • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|LicT regulon]
  • The protein

    Catalyzed reaction/ biological activity

  • Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to Swiss-Prot)
  • Protein family

  • glycosyl hydrolase 16 family (according to Swiss-Prot)
  • Structure

  • [PDB|3O5S]
  • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]
  • Expression and Regulation

    Operon

  • ''[protein|search|licT]-[protein|search|bglS]'' [Pubmed|8606172]
  • ''[protein|search|bglS]'' [Pubmed|8606172]
  • [SW|Sigma factor]

  • ''[protein|search|licT]'': [protein|search|SigA] [Pubmed|8606172]
  • Regulation

  • repressed by glucose (3.1-fold) ([protein|search|CcpA]) [Pubmed|12850135,8245831]
  • expressed in the stationary phase (temporal activation) [Pubmed|8245830]
  • weak induction by ß-glucosides ([protein|search|LicT]) [Pubmed|8606172]
  • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression [Pubmed|8245831]
  • [protein|search|LicT]: binding to an [SW|RNA switch] results in transcriptional antitermination [Pubmed|8606172]
  • Biological materials

    Mutant

  • GP427 (''[protein|search|licT]-[protein|search|bglS]'', erm), BGW7 (cat), both available in the [SW|Stülke] lab
  • References

  • 18957862,12850135,6087283,8245830,8245831,8606172,23459129,24391357,24659048,25808979