yobO

similar to phage-related pre-neck appendage protein

locus: BSU_19030

pI: 5.57

mw: 87.55 kDa

protein length: 806 aa

gene length: 2421 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 2,076,206 2,078,626

The protein

Structure

• [PDB|3SUC] (from phage Phi29, 40% identity) [pubmed|19450535]

Expression and Regulation

Operons

• genes: [gene|02269068E6E4282A32BB5AE0FAE27F104202E5B5|yobN]-[gene|032110CBA492D54F6494984323D12D719EAEAAD9|yobO]
  description: [Pubmed|22383849]

• genes: [gene|032110CBA492D54F6494984323D12D719EAEAAD9|yobO]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • subject to carbon catabolite repression ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|10666464]

Biological materials

Mutant

• MGNA-A312 (yobO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/312 NBRP B. subtilis, Japan]
• BKE19030 ([gene|032110CBA492D54F6494984323D12D719EAEAAD9|yobO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAAGACACCTCTTT, downstream forward: _UP4_TAGTGGATACGCCGACCAGT
• BKK19030 ([gene|032110CBA492D54F6494984323D12D719EAEAAD9|yobO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAAGACACCTCTTT, downstream forward: _UP4_TAGTGGATACGCCGACCAGT

References

• 20817675,10666464,19450535