mrnC

RNase Mini-III

locus: BSU_00950

pI: 9.15

mw: 16.09 kDa

protein length: 143 aa

gene length: 432 bp

function: maturation of 23S rRNA

product: RNase Mini-III

essential: no

synonyms: yazC

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

Gene

Coordinates: 114,854 115,285

The protein

Protein family

• MrnC RNase family (single member, according to UniProt)

Effectors of protein activity

• [protein|FEF94BD8482A41C493A8BEC3278B518E8BB4EDFE|RplC] binding to the precursor 23S rRNA stimulates MrnC activity [Pubmed|19154332]

Structure

• [PDB|4OUN] [pubmed|25634891]

Expression and Regulation

Operons

• genes: [gene|132E91DB8C81CD8C4F5160B2C068B74DA10AE224|gltX]-[gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]-[gene|E5826A99769E2FE45959A9D623D6467A8DDDEB81|cysS]-[gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]-[gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]-[gene|172652156ABFE72D5BAAC22729CE17CA947538B5|yacP]
  description: [Pubmed|7510287]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7510287], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|T-box|T-box]: transcription antitermination, overlaps a transcription terminator upstream of [gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE], in [regulon|T-box|T-box]

  regulation

  • expression transiently increases in the forespore [Pubmed|22848659]
  • the [SW|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-B883 (yazC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1882 NBRP B. subtilis, Japan]
• BKE00950 ([gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCGTATCAAATTCAAGCA, downstream forward: _UP4_TTCGGGACGTCAGGGAGGAA
• BKK00950 ([gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCGTATCAAATTCAAGCA, downstream forward: _UP4_TTCGGGACGTCAGGGAGGAA

labs

• [SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
• [SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]

References

• 19258532,18430137,18363798,8396117,19154332,19880604,25634891,27924926,32991829