lytS

two-component sensor kinase, control of pyruvate utilization

Locus: BSU_28930

Isoelectric point: 8.24

Molecular weight: 64.68 kDa

Protein length: 593 aa

Gene length: 1782 bp

Function: control of pyruvate utilization

Product: two-component sensor kinase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
• Information processing → Regulation of gene expression → Transcription factors and their control → Control of two-component response regulators → Two-component sensor kinase
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a His residue

Gene

Coordinates: 2,956,486 2,958,267

Phenotypes of a mutant

• no growth with pyruvate as the single carbon source PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-histidine --> ADP + N-phospho-L-histidine (according to UniProt)

Domains

• 5 transmembrane regions
• GAF domain (aa 238-363) (according to UniProt)
• Histidine kinase domain (aa 363-580) (according to UniProt)

Modification

• autophosphorylation on a His residue

Localization

• membrane (putative, due to presence of 5 membrane-spanning domains, according to Uniprot)

Expression and Regulation

Operons

• Genes: lytS-lytT
  Description: PubMed

Biological materials

Mutant

• MGNA-A015 (lytS::erm), available at the NBRP B. subtilis, Japan
• BKE28930 (lytS::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATGTCACCTGTTCTT, downstream forward: _UP4_GAACATGCTCAGGGTGTTAA
• BKK28930 (lytS::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCATGTCACCTGTTCTT, downstream forward: _UP4_GAACATGCTCAGGGTGTTAA

References

Reviews

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Original publications

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