lytS

two-component sensor kinase, control of pyruvate utilization

locus: BSU_28930

pI: 8.24

mw: 64.68 kDa

protein length: 593 aa

gene length: 1782 bp

function: control of pyruvate utilization

product: two-component sensor kinase

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 2,956,486 2,958,267

Phenotypes of a mutant

• no growth with pyruvate as the single carbon source [Pubmed|27422364]

The protein

Catalyzed reaction/ biological activity

• ATP + [protein|search|L-histidine] --> ADP + [protein|search|N-phospho-L-histidine] (according to UniProt)

[SW|Domains]

• 5 transmembrane regions
• [SW|GAF domain] (aa 238-363) (according to UniProt)
• [SW|Histidine kinase domain] (aa 363-580) (according to UniProt)

Modification

• autophosphorylation on a His residue

[SW|Localization]

• membrane (putative, due to presence of 5 membrane-spanning domains, according to Uniprot)

Expression and Regulation

Operons

• genes: [gene|02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD|lytS]-[gene|53BA24E91EEC797C95B8975C487F3C50CBBFA2E3|lytT]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A015 (lytS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/15 NBRP B. subtilis, Japan]
• BKE28930 ([gene|02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD|lytS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATGTCACCTGTTCTT, downstream forward: _UP4_GAACATGCTCAGGGTGTTAA
• BKK28930 ([gene|02FAD7D3A73CC0462D0116F4E5BA9505CBF7FCDD|lytS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATGTCACCTGTTCTT, downstream forward: _UP4_GAACATGCTCAGGGTGTTAA

References

Reviews

• 29208748,29354650

Original publications

• 10094672,27422364,28974613