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smc [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
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smc [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

part of the condensin complex, chromosomal origin condensation and segregation SMC protein
locus
BSU15940
pI
5.00
mw
135.00 kDa
function
segregation of replication origins
product
chromosome condensation and segregation SMC protein
essential
yes
synonyms
ylqA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [SW|Categories] containing this gene/protein

  • [SW|DNA condensation/ segregation], [SW|essential genes]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,666,560 -> 1,670,120

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • terminal phenotype of a CRISPRi knockdown mutant is moderate lysis [Pubmed|27238023]
  • ''[protein|search|smc]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]
  • ''[protein|search|smc]'' lethality on complex medim can be suppressed by inactivation of ''[protein|search|ylbM]'' and ''[protein|search|tsaC]'' (constitutive [SW|stringent response]) [Pubmed|26539825]
  • The protein

    Protein family

  • SMC family (according to Swiss-Prot)
  • Effectors of protein activity

  • ATP-induced DNA-binding results in a more open conformation of the [protein|search|Smc] dimer, and thus of the [SW|condensin] complex [Pubmed|25557547]
  • Structure

  • [PDB|3ZGX] (complex between the head domain of [protein|search|Smc] (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of [protein|search|ScpA]) [Pubmed|23353789]
  • [SW|Localization]

  • nucleoid (heterogeneous) [Pubmed|16479537]
  • the [SW|condensin] ([protein|search|Smc])2-[protein|search|ScpA]-[protein|search|ScpB] complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on [SW|Spo0J] [Pubmed|24440393,23475963]
  • [SW|Interactions]

  • part of the [SW|condensin] complex [Pubmed|24440399,24440393]
  • forms dimers [Pubmed|22385855]
  • [protein|search|ScpA]-[protein|search|Smc] [Pubmed|12100548]
  • [protein|search|ScpB]-[protein|search|Smc], [Pubmed|22385855,12100548,12421306], this interaction takes place only in the presence of [protein|search|ScpA] [Pubmed|22385855]
  • [protein|search|Smc] is a member of a suspected group of hubs proteins that were suggested to be involved in a large number of [SW|interactions] [Pubmed|21630458]
  • Expression and Regulation

    Operon

  • ''[protein|search|rnc]-[protein|search|smc]-[protein|search|ftsY]'' [Pubmed|11021934]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|11021934]
  • Biological materials

    Mutant

  • 1A822 ( ''smc''::pMUTIN ''trpC2'' Em), [Pubmed|12682299], available at [http://bgsc.org/getdetail.php?bgscid=1A822 the Bacillus Genetic Stock Center]
  • BEC15940 (''smc''::''erm trpC2''), a CRISPRi knockdown mutant, [Pubmed|27238023], is available at the [http://bgsc.org Bacillus Genetic Stock Center]
  • References

    Reviews

  • 19308706,22934648,22047950,22933559,24118085,25460803,26706151
  • Original publications

  • 27238023,9791128,12100548,17416649,19450516,12421306,11395470,19450517,19450516,15009890,16479537,21630458,23353789,25071173,22385855,25951515,26253537,24440399,24440393,23475963,25557547,25664627,26295962,26539825,26725510,26904953