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spoIIIAH [Mon Oct 10 2016 16:45:46 GMT+0200 (CEST)]
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spoIIIAH [Mon Oct 10 2016 16:45:46 GMT+0200 (CEST)]

component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation
locus
BSU24360
pI
4.00
mw
23.00 kDa
function
activation of SigG, forespore encasement by the spore coat
product
part of the transmembrane channel linking the mother cell and the forespore
essential
no
synonyms
Outlinks
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Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|protein secretion], [SW|sporulation proteins], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|SigE regulon], [SW|SpoIIID regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,532,353 -> 2,533,009

    Phenotype of a mutant

  • reduced sporulation efficiency (1 to 10% compared to wild type) [Pubmed|26735940]
  • the ''[SW|spoIIIL] [SW|spoIIIAH]'' double mutant has a severe sporulation defect (0.001%) [Pubmed|26735940]
  • block of sporulation after engulfment

    • The protein

    Catalyzed reaction/ biological activity

  • required for forespore encasement by the spore coat [Pubmed|22171814]

    • Structure

  • [PDB|3UZO]; [PDB|3TUF] (the [SW|SpoIIQ]-[SW|SpoIIIAH] pore forming complex) [Pubmed|22431604,22431613]

    • [SW|Localization]

  • membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with [SW|SpoIIQ]) [Pubmed|22431604,22431613,22171814,18485064]
  • proper recruitment to the sporulation septum on the mother cell side requires [SW|SpoIIQ] [Pubmed|23834622]

    • [SW|Interactions]

  • [SW|SpoIIIAH]-[SW|SpoIIQ] (interaction between proteins membrane proteins expressed in the mother cell and the forespore, respectively; the complex forms a 60 Å channel made up of twelve subunits of each protein) [Pubmed|22431604,22431613,15574594,21097616]
  • [SW|GerM]-[SW|SpoIIIAH] [Pubmed|27381174]

    • Expression and Regulation

    Operon

  • ''[SW|spoIIIAA]-[SW|spoIIIAB]-[SW|spoIIIAC]-[SW|spoIIIAD]-[SW|spoIIIAE]-[SW|spoIIIAF]-[SW|spoIIIAG]-[SW|spoIIIAH]'' [Pubmed|18485064]
  • ''[SW|spoIIIAG]-[SW|spoIIIAH]'' [Pubmed|17693505]

    • [SW|Sigma factor]

  • [protein|search|SigE] [Pubmed|15699190]
  • both promoters: [protein|search|SigE] [Pubmed|18485064]

    • Regulation

  • both promoters: expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|18485064], switched off by [SW|SpoIIID] [Pubmed|15383836]

    • Regulatory mechanism

  • [SW|SpoIIID]: transcription repression [Pubmed|15383836]

    • Additional information

  • the internal promoter is essential for sporulation, it is twice as active as the promoter in front of [protein|search|spoIIIAA], suggesting that [protein|search|SpoIIIAG] and [protein|search|SpoIIIAH] are required in larger amounts as compared to the other products of the operon [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed]

    • Labs working on this gene/protein

  • [SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

    • References

    Reviews

  • 23944268,25105965

    • Original publications

  • 15752199,18485064,15574594,18812514,1766372,17693505,19609349,17121846,21097616,22431613,22171814,23834622,22431604,23859254,25356555,26735940,27381174