niaP

nicotinate transporter

locus: BSU_02950

pI: 9.50

mw: 43.55 kDa

protein length: 400 aa

gene length: 1203 bp

function: uptake of niacin

product: nicotinate transporter

essential: no

synonyms: yceI

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.7|Transporter for cofactors]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.11|Biosynthesis of NAD(P)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 317,725 318,927

Phenotypes of a mutant

• more sensitive to nisin [Pubmed|23980836]

The protein

Protein family

• [SW|major facilitator superfamily] [Pubmed|18276644]
• [SW|Sugar transporter (TC 2.A.1.1) family] (according to UniProt)

[SW|Domains]

• 10 membrane-spanning domains [Pubmed|18276644]

Structure

• [PDB|4J05] (from ''P. indica'', 24% identity) [Pubmed|23542591]

[SW|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|0037A6808E75C2A2E207A7005EFDA71FCE90BAD8|niaP]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|510DE075EA964D22C42DEB171509F6CDA600A402|NadR]: repression, [Pubmed|18276644], in [regulon|510DE075EA964D22C42DEB171509F6CDA600A402|NadR regulon]

  regulation

  • repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|18276644]

Biological materials

Mutant

• MGNA-C047 (yceI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2045 NBRP B. subtilis, Japan]
• BKE02950 ([gene|0037A6808E75C2A2E207A7005EFDA71FCE90BAD8|niaP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCCAACACCTCTGATA, downstream forward: _UP4_TAGGAAAAGCACCTCTTAAA
• BKK02950 ([gene|0037A6808E75C2A2E207A7005EFDA71FCE90BAD8|niaP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCCAACACCTCTGATA, downstream forward: _UP4_TAGGAAAAGCACCTCTTAAA

References

Reviews

• 21953179

Original publications

• 18763711,18276644,23980836,22383849,23542591