SubtiBank SubtiBank
yxaL
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yxaL

similar to Ser/Thr kinase, increases the processivity of the PcrA helicase
Locus
BSU_39940
Isoelectric point
6.38
Molecular weight
43.72 kDa
Protein length
410 aa Sequence Blast
Gene length
1233 bp Sequence Blast
Function
unknown
Product
unknown
Essential
no
Synonyms
yxaK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Groups of genes → Secreted proteins

    • Gene

    Coordinates
    4,102,429 4,103,661

    The protein

    Structure

  • 3HXJ (from Methanococcus maripaludis, 33% Identity)

    • Localization

  • extracellular (signal peptide), major constituent of the secretome PubMed

    • Expression and Regulation

    Operons

    Genes
    yxaJ-yxaL
    Description
    PubMed

    Regulatory mechanism

  • Rok: repression, PubMed, in Rok regulon
  • DnaA: repression, Rok-DnaA PubMed, in DnaA regulon
  • AbrB: repression, PubMed, in AbrB regulon

    • Regulation

  • repressed by Rok PubMed
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    Biological materials

    Mutant

  • MGNA-B687 (yxaL::erm), available at the NBRP B. subtilis, Japan
  • BKE39940 (yxaL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATTGAGACCCCCATTA, downstream forward: _UP4_TAACTGAATAGAATAGCAGA
  • BKK39940 (yxaL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATTGAGACCCCCATTA, downstream forward: _UP4_TAACTGAATAGAATAGCAGA

    • Expression vectors

  • pGP392 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab
  • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP824, available in Jörg Stülke's lab, PubMed

    • References

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