Modulator of [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN] activity, similar to phosphoenolpyruvate synthetase regulatory protein
function
inhibits [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN] activity
product
modulator of [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN] activity
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]Gene
Coordinates
2,604,121 → 2,604,933
The protein
Catalyzed reaction/ biological activity
negative effector of [protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|CcpN] activity [Pubmed|15720552]ADP + Nτ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] --> AMP + H+ + Nτ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)H+ + Nτ-phospho-L-histidyl/O-phospho-L-threonyl-[pyruvate, phosphate dikinase] + phosphate --> diphosphate + Nτ-phospho-L-histidyl/L-threonyl-[pyruvate, phosphate dikinase] (according to UniProt)Protein family
pyruvate, phosphate/water dikinase regulatory protein family (single member, according to UniProt)[SW|Domains]
nucleotide binding domain (ATP) (151–158)Structure
[PDB|5D0N] (from maize, 39% identity) [pubmed|26620526]Expression and Regulation
Operons
genes
[gene|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN]-[gene|B3C0FE9DD3CB4AF34DD57CF4D03ABE8AFF1346C7|yqfL]
description
[Pubmed|15720552]
regulation
constitutive [Pubmed|15720552]additional information
the intracellular concentration of CcpN is about 4 myM (according to [PubMed|20408793]).view in new tabBiological materials
Mutant
MGNA-C492 (yqfL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2490 NBRP B. subtilis, Japan]BKE25240 (Δ[gene|B3C0FE9DD3CB4AF34DD57CF4D03ABE8AFF1346C7|yqfL]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCCCCGTTAGT, downstream forward: _UP4_TAACTCAGGACGCTCTATCCBKK25240 (Δ[gene|B3C0FE9DD3CB4AF34DD57CF4D03ABE8AFF1346C7|yqfL]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTATCCCCCCGTTAGT, downstream forward: _UP4_TAACTCAGGACGCTCTATCClabs
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, FranceReferences
15720552,26620526,20044937