SubtiBank SubtiBank
ykfB
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ykfB

L-Ala-D/L-Glu epimerase
Locus
BSU_12980
Isoelectric point
5.91
Molecular weight
39.31 kDa
Protein length
366 aa Sequence Blast
Gene length
1101 bp Sequence Blast
Function
cell wall metabolism
Product
L-Ala-D/L-Glu epimerase
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall/ other

    • Gene

    Coordinates
    1,366,844 1,367,944

    The protein

    Catalyzed reaction/ biological activity

  • L-alanyl-L-glutamate --> L-alanyl-D-glutamate (according to UniProt)

    • Protein family

  • mandelate racemase/muconate lactonizing enzyme family (according to UniProt)

    • Structure

  • 1TKK (complex with substrate), 1JPM

    • Expression and Regulation

    Operons

    Genes
    dppA-dppB-dppC-dppD-dppE-ykfA-ykfB-ykfC-ykfD
    Description
    PubMed

    Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
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    Biological materials

    Mutant

  • MGNA-A741 (ykfB::erm), available at the NBRP B. subtilis, Japan
  • BKE12980 (ykfB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCTTGTTTCGATTCGAATGA, downstream forward: _UP4_CTTTTGAAAGGGGAAAAAGA
  • BKK12980 (ykfB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GCTTGTTTCGATTCGAATGA, downstream forward: _UP4_CTTTTGAAAGGGGAAAAAGA

    • References

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