SubtiBank SubtiBank
yfmT
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yfmT

vanillin dehydrogenase
Locus
BSU_07350
Isoelectric point
5.08
Molecular weight
53.15 kDa
Protein length
485 aa Sequence Blast
Gene length
1458 bp Sequence Blast
Function
unknown
Product
vanillin dehydrogenase
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Groups of genes → Poorly characterized/ putative enzymes

    • Gene

    Coordinates
    807,091 → 808,548

    The protein

    Catalyzed reaction/ biological activity

  • oxidation of vanillin to vanillic acid PubMed
  • benzaldehyde + H2O + NAD+ --> benzoate + 2 H+ + NADH (according to UniProt)
  • 4-hydroxy-3-methoxybenzaldehyde + H2O + NAD+ --> 4-hydroxy-3-methoxybenzoate + 2 H+ + NADH (according to UniProt)

    • Protein family

  • aldehyde dehydrogenase family (according to UniProt)

    • Paralogous protein(s)

  • AldX, AldY, GbsA, YcbD, DhaS, IolA, YwdH, GabD, PutC, RocA

    • Structure

  • 4DNG (from Bacillus Subtilis, 47% identity)
  • 3RHH (from from Bacillus halodurans C-125 complexed with NADP, 32% identity, 67% similarity)

    • Expression and Regulation

    Operons

    Genes
    yfmT-yfmS
    Description
    PubMed

    Sigma factors

  • SigD: sigma factor, PubMed, in SigD regulon

    • Additional information

  • in minimal medium, YfmS is present with 4,200 +/- 800 molecules per cell PubMed
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    Biological materials

    Mutant

  • MGNA-C232 (yfmT::erm), available at the NBRP B. subtilis, Japan
  • BKE07350 (ΔyfmT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTCAGCATCTCCTTTA, downstream forward: _UP4_TTCCCTTATTAATGAAAAGG
  • BKK07350 (ΔyfmT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTCAGCATCTCCTTTA, downstream forward: _UP4_TTCCCTTATTAATGAAAAGG

    • Labs working on this gene/protein

  • Josef Altenbuchner

    • References

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