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ycdF
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ycdF

general stress protein, similar to glucose 1-dehydrogenase, survival of ethanol stress and low temperatures
Locus
BSU_02830
Isoelectric point
5.74
Molecular weight
27.62 kDa
Protein length
258 aa Sequence Blast
Gene length
777 bp Sequence Blast
Function
survival of ethanol stress and low temperatures
Product
unknown
Essential
no
E.C.
1.1.1.47
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
  • Groups of genes → Poorly characterized/ putative enzymes

    • Gene

    Coordinates
    305,658 → 306,434

    The protein

    Catalyzed reaction/ biological activity

  • Beta-D-glucose + NAD(P)+ --> D-glucono-1,5-lactone + NAD(P)H (according to UniProt)
  • Beta-D-glucose + NAD+ --> D-glucono-1,5-lactone + NADH (according to UniProt)

    • Protein family

  • Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)

    • Paralogous protein(s)

  • YxbG, BacC, YmfI, FabG, YoxD
  • Gdh:
  • FadH:
  • YhdF:
  • KduD:
  • YdaD:
  • YkvO:
  • YhxC:
  • YhxD:
  • FabL:

    • Structure

  • 1GEE (from Bacillus megaterium, 56% identity)

    • Expression and Regulation

    Operons

    Genes
    ycdF-ycdG
    Description
    PubMed

    Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

    • Regulation

  • induced by stress (SigB) PubMed
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    Biological materials

    Mutant

  • MGNA-C046 (ycdF::erm), available at the NBRP B. subtilis, Japan
  • BKE02830 (ΔycdF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTTGATATCCTCCTCT, downstream forward: _UP4_TAGAAGACAAAACAGGGGGA
  • BKK02830 (ΔycdF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTTGATATCCTCCTCT, downstream forward: _UP4_TAGAAGACAAAACAGGGGGA

    • References

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