SubtiBank SubtiBank
trmN6
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trmN6

tRNA methyltransferase
Locus
BSU_00340
Isoelectric point
6.50
Molecular weight
28.13 kDa
Protein length
247 aa Sequence Blast
Gene length
744 bp Sequence Blast
Function
tRNA modification
Product
tRNA methyltransferase
Essential
no
Synonyms
yabB

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
42,917 43,660

The protein

Structure

  • 3LPM (putative methyltransferase from Listeria monocytogenes, 64% identity)
  • Expression and Regulation

    Operons

    Description

    Regulation

  • expressed during growth and the transition phase, expression is erduced in stationary phase PubMed
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    Biological materials

    Mutant

  • MGNA-B901 (yabB::erm), available at the NBRP B. subtilis, Japan
  • BKE00340 (trmN6::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTCTTATCCTTTCAGT, downstream forward: _UP4_ACAAAAGAAATCAGGACCAT
  • BKK00340 (trmN6::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTCTTATCCTTTCAGT, downstream forward: _UP4_ACAAAAGAAATCAGGACCAT
  • References

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