SubtiBank SubtiBank
walJ
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walJ

5'-3' double-stranded DNA exonuclease, coordination of cell division with DNA replication
Locus
BSU_40370
Isoelectric point
5.50
Molecular weight
29.77 kDa
Protein length
264 aa Sequence Blast
Gene length
795 bp Sequence Blast
Function
coordination of cell division with DNA replication
Product
5'-3' double-stranded DNA exonuclease
Essential
no
E.C.
3.-.-.-
Synonyms
yycJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of peptidoglycan
  • Cellular processes → Cell envelope and cell division → Cell division → Other genes
  • Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of peptidoglycan
  • Information processing → Genetics → DNA repair/ recombination → Mismatch repair (MMR)

    • Gene

    Coordinates
    4,148,851 → 4,149,645

    Phenotypes of a mutant

  • the mutant divides over unsegregated chromosomes more frequently than wild-type cells, and this phenotype is exacerbated when DNA replication is inhibited PubMed
  • increased sensitivity to a narrow spectrum of cephalosporin antibiotics PubMed
  • disruption of the corresponding gene in B. anthracis results in a mutator phenotype PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • 5'-3' exonuclease activity at the 5' termini and at nicks of double-stranded DNA PubMed
  • the enzymatic activity of WalJ directly or indirectly affects cell wall metabolism and is required for accurate coordination of cell division with DNA replication PubMed

    • Protein family

  • predicted member of the metallo-β-lactamase superfamily

    • Cofactors

  • Mn(II) PubMed

    • Expression and Regulation

    Operons

    Genes
    walR-walK-walH-walI-walJ-htrC
    Description
    PubMed

    Regulation

  • htrC transcript expressed during sporulation PubMed
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    Biological materials

    Mutant

  • GP3558 (ΔwalJ::aphA3), available in Jörg Stülke's lab
  • MGNA-B824 (yycJ::erm), available at the NBRP B. subtilis, Japan
  • 1A1044 ( walJ::spec), PubMed, available at [BGhttp://bgsc.org/getdetail.php?bgscid=1A1044]
  • BKE40370 (ΔwalJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTGTCTCACTCCATT, downstream forward: _UP4_TAATTTTTCTATGTAAATCA
  • BKK40370 (ΔwalJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTGTCTCACTCCATT, downstream forward: _UP4_TAATTTTTCTATGTAAATCA

    • References

    Reviews

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    Original publications

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